Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation

被引：21

作者：

Murakami, Kenji ^{[1
]}

Gibbons, Brian J. ^{[1
]}

Davis, Ralph E. ^{[1
]}

Nagai, Shigeki ^{[1
]}

Liu, Xin ^{[1
]}

Robinson, Philip J. J. ^{[1
]}

Wu, Tinghe ^{[1
]}

Kaplan, Craig D. ^{[2
]}

Kornberg, Roger D. ^{[1
]}

机构：

[1] Stanford Univ, Dept Biol Struct, Stanford, CA 94305 USA

[2] Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2012年 / 109卷 / 13期

关键词：

DNA repair; general transcription factor; RNA polymerase II; TFIIK; transcription pre-initiation complex; yeast; RNA-POLYMERASE-II; DNA-REPAIR; MESSENGER-RNA; COMPONENT; COMPLEX; KINASE;

D O I：

10.1073/pnas.1201448109

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

General transcription factor TFIIH, previously described as a 10-subunit complex, is essential for transcription and DNA repair. An eleventh subunit now identified, termed Tfb6, exhibits 45% sequence similarity to human nuclear mRNA export factor 5. Tfb6 dissociates from TFIIH as a heterodimer with the Ssl2 subunit, a DNA helicase that drives promoter melting for the initiation of transcription. Tfb6 does not, however, dissociate Ssl2 from TFIIH in the context of a fully assembled transcription preinitiation complex. Our findings suggest a dynamic state of Ssl2, allowing its engagement in multiple cellular processes.

引用

页码：4816 / 4821

页数：6

共 4 条

[1] Systematic mutagenesis of TFIIH subunit p52/Tfb2 identifies residues required for XPB/Ssl2 subunit function and genetic interactions with TFB6
Bassett, Jacob
Rimel, Jenna K.
Basu, Shrabani
Basnet, Pratik
Luo, Jie
Engel, Krysta L.
Nagel, Michael
Woyciehowsky, Alexander
Ebmeier, Christopher C.
Kaplan, Craig D.
Taatjes, Dylan J.
Ranish, Jeffrey A.
JOURNAL OF BIOLOGICAL CHEMISTRY, 2022, 298 (10)
[2] The mouse p52 subunit of the transcription/DNA repair factor TFIIH is not located in the class III region of the H2 complex, but resides next to a G7a/Bat6 homologue in the telomeric part of the major histocompatibility complex
M. Snoek
M. van Kooij
K. de Groot
H. van Vugt
Immunogenetics, 2000, 51 : 164 - 166
[3] The mouse p52 subunit of the transcription/DNA repair factor TFIIH is not located in the class III region of the H2 complex, but resides next to a G7a/Bat6 homologue in the telomeric part of the major histocompatibility complex
Snoek, M
van Kooij, M
de Groot, K
van Vugt, H
IMMUNOGENETICS, 2000, 51 (02) : 164 - 166
[4] The mouse p52 subunit of the transcription/DNA repair factor TFIIH is not located in the class III region of the H2 complex, but resides next to a G7a/Bat6 homologue in the telomeric part of the major histocompatibility complex -: Response
Lafuse, W
IMMUNOGENETICS, 2000, 51 (02) : 167 - 167

← 1 →