ox-LDL induces endothelial dysfunction by promoting Arp2/3 complex expression

被引：20
|
作者：
Tang, Yao [1 ]
Zhao, Jianting [1 ]
Shen, Liming [1 ]
Jin, Yiqi [1 ]
Zhang, Zhixuan [1 ]
Xu, Guoxiong [1 ]
Huang, Xianchen [1 ]
机构：
[1] Nanjing Med Univ, Suzhou Hosp Affiliated, Suzhou Municipal Hosp, Vasc Surg, 26 Daoqian St, Suzhou 215002, Jiangsu, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2016年 / 475卷 / 02期
关键词：
Arp2/3; ox-LDL; LOX-1; Rac-1; Endothelial dysfunction; LOW-DENSITY-LIPOPROTEIN; CELL-SHAPE; ACTIN; ATHEROSCLEROSIS; ATHEROGENESIS; ACTIVATION; GAMMA;
D O I：
10.1016/j.bbrc.2016.05.068
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Oxidized low-density lipoproteins (ox-LDL) play a critical role in endothelial injury including cytoskeleton reorganization, which is closely related to actin-related protein 2/3 (Arp2/3) complex. The aim of this study was to investigate the role of Arp2/3 complex in ox-LDL-induced endothelial dysfunction. In this study, we found that Arp2 and Arp3 expression was increased under atherosclerotic conditions both in ApoE(-/-) mice and in ox-LDL-stimulated human coronary artery endothelial cells (HCAECs). Arp2/3 complex inhibitor CK666 significantly reduced ox-LDL-induced ROS generation and cytoskeleton reorganization, and increased NO release in HCAECs. Pretreatment with LOX-1- but not CD36-blocking antibody markedly decreased ox-LDL-induced Arp2 and Arp3 expression. Moreover, Rac-1 siRNA remarkably suppressed ox-LDL-stimulated Arp2 and Arp3 expression. Additionally, CK666 reduced endothelial nitric oxide synthase (eNOS) expression and atherosclerotic lesions in ApoE(-/-) mice. Collectively, ox-LDL induces endothelial dysfunction by activating LOX-1/Rac-1 signaling and upregulating Arp2/3 complex expression. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码：182 / 188
页数：7
相关论文
共 50 条
  • [1] Ox-LDL Induces Dysfunction of Endothelial Progenitor Cells via Activation of NF-κB
    Ji, Kang-ting
    Qian, Lu
    Nan, Jin-liang
    Xue, Yang-jing
    Zhang, Su-qin
    Wang, Guo-qiang
    Yin, Ri-peng
    Zhu, Yong-jin
    Wang, Lu-ping
    Ma, Jun
    Liao, Lian-ming
    Tang, Ji-fei
    BIOMED RESEARCH INTERNATIONAL, 2015, 2015
  • [2] Activation of the Arp2/3 complex by nucleation promoting factors
    Benanti, E. L.
    Siripala, A. D.
    Znameroski, E. A.
    Welch, M. D.
    MOLECULAR BIOLOGY OF THE CELL, 2011, 22
  • [3] Mechanism of Activation of the Arp2/3 Complex by Nucleation Promoting Factors
    Boczkowska, M.
    Rebowski, G.
    Dominguez, R.
    MOLECULAR BIOLOGY OF THE CELL, 2012, 23
  • [4] Mechanism of Activation of the Arp2/3 Complex by Nucleation Promoting Factors
    Rebowski, Grzegorz
    Boczkowska, Malgorzata
    Dominguez, Roberto
    BIOPHYSICAL JOURNAL, 2013, 104 (02) : 139A - 139A
  • [5] The mechanism of Arp2/3 complex activation by Nucleation Promoting Factors (NPFs)
    Zalevsky, J
    Kelly, AE
    Mullins, RD
    MOLECULAR BIOLOGY OF THE CELL, 2000, 11 : 379A - 379A
  • [6] The mechanism of Arp2/3 complex activation by nucleation promoting factors (NPFs)
    Kelly, AE
    Zalevsky, J
    Mullins, RD
    MOLECULAR BIOLOGY OF THE CELL, 2001, 12 : 289A - 289A
  • [7] Phosphorylation of Arp2 is not essential for Arp2/3 complex activity in fission yeast
    Epstein, Alexander E.
    Espinoza-Sanchez, Sofia
    Pollard, Thomas D.
    LIFE SCIENCE ALLIANCE, 2018, 1 (05)
  • [8] Analysis of microRNA expression profile in apoptotic endothelial cells induced by ox-LDL
    Qin, Bing
    Lu, Zhengqi
    JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2018, 72 (16) : C78 - C78
  • [9] Nucleation Promoting Factors Regulate the Expression and Localization of Arp2/3 Complex during Meiosis of Mouse Oocytes
    Liu, Jun
    Wang, Qiao-Chu
    Wang, Fei
    Duan, Xing
    Dai, Xiao-Xin
    Wang, Teng
    Liu, Hong-Lin
    Cui, Xiang-Shun
    Kim, Nam-Hyung
    Sun, Shao-Chen
    PLOS ONE, 2012, 7 (12):
  • [10] Arp2/3 complex function in the epidermis
    Lechler, Terry
    TISSUE BARRIERS, 2014, 2 (04):
12345