Submerged Culture Medium Composition for the Antioxidant Activity by Grifola frondosa TFRI1073

Effects of nutritional requirements for superoxide anion scavenging activity and reducing power by a newly screened Grifola frondosa TFRI1073 were studied in submerged cultures. The results clearly showed that the culture medium significantly affected superoxide anion scavenging activity and reducing power. In addition, both superoxide anion scavenging capability and reducing power were found to be greatly affected by varying the carbon source, the nitrogen source, the growth factor, and the mineral elements. The best superoxide anion scavenging activity could be obtained when the medium compositions (carbon source, nitrogen source, growth factor, and the mineral elements) were sucrose, ammonium chloride, nicotinic acid, K3PO4, and CuSO4; while a maximal reducing power could be achieved when the medium composition setting as: xylose, arginine, ascorbic acid, K2HPO4, and MgSO4. From a series experiments, the maximal superoxide anion scavenging activity and reducing power were 98.36 +/- 0.59% and 0.947 +/- 0.06 at 100 mu g/mL, respectively. This study proved that modifying the composition of the medium can dramatically enhance the superoxide anion scavenging ability and reducing power by a newly screened G frondosa TFRI1073.