Identification of the Rhizobium meliloti alcohol dehydrogenase gene (adhA) and heterologous expression in Alcaligenes eutrophus

被引：9
|
作者：
Willis, LB [1 ]
Walker, GC [1 ]
机构：
[1] MIT, Dept Biol, Cambridge, MA 02139 USA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1998年 / 1384卷 / 02期
关键词：
alcohol dehydrogenase; poly-beta-hydroxybutyrate; (Rhizobium meliloti); (Alcaligenes eutrophus);
D O I：
10.1016/S0167-4838(98)00048-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A screen for Rhizobium meliloti genes which improve the growth of Alcaligenes eutrophus on sucrose identified the first alcohol dehydrogenase gene (adhA) isolated from the Rhizobiaceae. R, meliloti adhA is constitutively expressed in A. eutrophus and has alcohol dehydrogenase activity. R. meliloti adhA mutants retain some alcohol dehydrogenase activity. (C) 1998 Elsevier Science B.V. All rights reserved.
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页码：197 / 203
页数：7
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