Genetic and Phytochemical Characterization of Lettuce Flavonoid Biosynthesis Mutants

被引:20
|
作者
Gurdon, Csanad [1 ]
Poulev, Alexander [1 ]
Armas, Isabel [1 ]
Satorov, Shukhratdzhon [1 ]
Tsai, Meg [1 ]
Raskin, Ilya [1 ]
机构
[1] Rutgers State Univ, Dept Plant Biol, 59 Dudley Rd, New Brunswick, NJ 08901 USA
基金
美国国家卫生研究院;
关键词
CORONARY-HEART-DISEASE; POTENTIALLY ANTICARCINOGENIC FLAVONOIDS; IMPROVES GLUCOSE-METABOLISM; CHALCONE ISOMERASE ACTIVITY; NARINGENIN CHALCONE; ULTRAVIOLET-RADIATION; 3'-HYDROXYLASE GENE; LACTUCA-SATIVA; LOLLO-ROSSO; VEGETABLES;
D O I
10.1038/s41598-019-39287-y
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We previously developed red lettuce (Lactuca sativa L.) cultivars with high flavonoid and phenolic acid content and demonstrated their anti-diabetic effect. Here we report on developing three fertile and true-breeding lettuce lines enriched with flavonoids with reported beneficial health effects. These lines were identified in a segregating population of EMS-mutagenized red lettuce and characterized biochemically and genetically. Change in red coloration was used as a visual indicator of a mutation in a flavonoid pathway gene, leading to accumulation of flavonoid precursors of red anthocyanins. Pinkgreen kaempferol overproducing kfoA and kfoB mutants accumulated kaempferol to 0.6-1% of their dry weight, higher than in any vegetable reported. The yellow-green naringenin chalcone overproducing mutant (nco) accumulated naringenin chalcone, not previously reported in lettuce, to 1% dry weight, a level only observed in tomato peel. Kfo plants carried a mutation in the FLAVONOID-3' HYDROXYLASE (F3' H) gene, nco in CHALCONE ISOMERASE (CHI). This work demonstrates how non-GMO approaches can transform a common crop plant into a functional food with possible health benefits.
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页数:12
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