MiR-645 promotes invasiveness, metastasis and tumor growth in colorectal cancer by targeting EFNA5

被引:30
|
作者
Li, Shuai [1 ]
Hou, Xinfang [1 ]
Wu, Chen [1 ]
Han, Lili [1 ]
Li, Qian [1 ]
Wang, Jufeng [1 ]
Luo, Suxia [1 ]
机构
[1] Zhengzhou Univ, Affiliated Canc Hosp, Henan Canc Hosp, Dept Internal Med, 127 Dongming Rd, Zhengzhou 450008, Henan, Peoples R China
关键词
MiR-645; EFNA5; Cell invasion; Metastasis; Colorectal cancer; HEPATOCELLULAR-CARCINOMA; PROGRESSION; MIR-20A; GLIOMA;
D O I
10.1016/j.biopha.2020.109889
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
MicroRNA-645 (miR-645) has been implicated in numerous types of human cancers including colon cancer. However, the effects and mechanisms of action of miR-645 dysregulation on the growth and malignancy of colorectal cancer (CRC) remain unclear. In this study, we demonstrated that miR-645 knockdown significantly diminished CRC cell migration and invasion and repressed epithelial-mesenchymal transition (EMT). Conversely, miR-645 overexpression enhanced CRC cell migration, invasion, and EMT. In vivo assays confirmed that miR-645 knockdown substantially reduced CRC growth and metastasis. Regarding the mechanism, ephrinA5 (EFNA5) was identified as a direct target gene of miR-645. MiR-645 specifically targeted the 3'-untranslated region of EFNA5 mRNA and hindered its expression. EFNA5 knockdown attenuated the effects of miR-645 knockdown on CRC cell migration and invasion. Additionally, we noted a statistically significant inverse correlation between EFNA5 mRNA and miR-645 levels in tumors from 28 patients with CRC. Hence, miR-645 acts as an oncogenic miRNA that may increase CRC cell migration, invasiveness, and metastasis by targeting EFNA5.
引用
收藏
页数:7
相关论文
共 50 条
  • [1] EFNA5 suppresses cell proliferation and tumor metastasis in hepatoma via epithelial-to-mesenchymal transition
    Zhu, Zhiqin
    Hu, Shulu
    Zhong, Xingyi
    Zhang, Yangfeng
    Wu, Xiuqiong
    Lin, Junhao
    Chen, Fengsheng
    DISCOVER ONCOLOGY, 2024, 15 (01)
  • [2] miR-196a-5p promotes metastasis of colorectal cancer via targeting IB
    Xin, He
    Wang, Chuanzhuo
    Liu, Zhaoyu
    BMC CANCER, 2019, 19 (1)
  • [3] miR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα
    He Xin
    Chuanzhuo Wang
    Zhaoyu Liu
    BMC Cancer, 19
  • [4] MiR-645 promotes proliferation and migration of non-small cell lung cancer cells by targeting TP53I11
    Zhu, B.
    Tian, T.
    Zhao, M.
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2020, 24 (11) : 6150 - 6156
  • [5] CREB5 promotes invasiveness and metastasis in colorectal cancer by directly activating MET
    Shuyang Wang
    Junfeng Qiu
    Lei Liu
    Cailin Su
    Lu Qi
    Chengmei Huang
    Xiaoning Chen
    Yaxin Zhang
    Yaping Ye
    Yanqing Ding
    Li Liang
    Wenting Liao
    Journal of Experimental & Clinical Cancer Research, 39
  • [6] CREB5 promotes invasiveness and metastasis in colorectal cancer by directly activating MET
    Wang, Shuyang
    Qiu, Junfeng
    Liu, Lei
    Su, Cailin
    Qi, Lu
    Huang, Chengmei
    Chen, Xiaoning
    Zhang, Yaxin
    Ye, Yaping
    Ding, Yanqing
    Liang, Li
    Liao, Wenting
    JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2020, 39 (01)
  • [7] MiR-198 represses tumor growth and metastasis in colorectal cancer by targeting fucosyl transferase 8
    Wang, Minyu
    Wang, Jilin
    Kong, Xuan
    Chen, Huimin
    Wang, Yingchao
    Qin, Miao
    Lin, Yanwei
    Chen, Haoyan
    Xu, Jie
    Hong, Jie
    Chen, Ying-Xuan
    Zou, Weiping
    Fang, Jing-Yuan
    SCIENTIFIC REPORTS, 2014, 4
  • [8] MiR-198 represses tumor growth and metastasis in colorectal cancer by targeting fucosyl transferase 8
    Minyu Wang
    Jilin Wang
    Xuan Kong
    Huimin Chen
    Yingchao Wang
    Miao Qin
    Yanwei Lin
    Haoyan Chen
    Jie Xu
    Jie Hong
    Ying-Xuan Chen
    Weiping Zou
    Jing-Yuan Fang
    Scientific Reports, 4
  • [9] Propofol postpones colorectal cancer development through circ_0026344/miR-645/Akt/mTOR signal pathway
    Cui, Xiaomin
    Feng, Jiying
    Wu, Jian
    Zhang, Xiaobao
    Ding, Mengyao
    OPEN MEDICINE, 2021, 16 (01): : 570 - 580
  • [10] CircFAM120B Blocks the Development of Colorectal Cancer by Activating TGF-Beta Receptor II Expression via Targeting miR-645
    Yu, You
    Lei, Xiao
    FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2021, 9