Antisense transgenesis of tobacco with a flax pectin methylesterase affects pollen ornamentation

被引：16
|
作者：
Lacoux, J
Gutierrez, L
Dantin, F
Beaudoin, B
Roger, D
Lainé, E
机构：
[1] Univ Picardie Jules Verne, Fac Sci, Lab Biotechnol & Physiol Vegetales, F-80039 Amiens, France
[2] Univ Picardie Jules Verne, Fac Sci, Lab React & Chim Solides, F-80039 Amiens, France
来源：
PROTOPLASMA | 2003年 / 222卷 / 3-4期
关键词：
Linum usitatissimum; Nicotiana tabacum; exine; scanning electron microscopy;
D O I：
10.1007/s00709-003-0019-1
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Antisense transgenesis of tobacco (Nicotiana tabacum) with a partial flax (Linum usitatissimum L.) pectin methylesterase (Lupme3) cDNA sequence yielded plants with altered pollen content. Moreover, the characteristically sculptured cell wall surrounding the pollen grains was modified in transgenic tobacco plants: the wavy ornamentation was dramatically reduced, suggesting the involvement of the demethylation of pectin in the pollen cell wall-specific structure. Germination of pollen was decreased and the pollen tube surface aspect was also different in transgenic plants.
引用
收藏
页码：205 / 209
页数：5
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