The groESL ISR sequence-based species-specific identification of GRAS and non-GRAS Lactiplantibacillus as an alternative to 16S rRNA sequencing

被引：3
|
作者：
Lee, Jong Min [1 ,2 ]
Park, So Hee [2 ,3 ]
Jin, Chun-Zhi [2 ]
Kang, Min-Kyoung [2 ]
Park, Dong-Jin [2 ]
Kim, Chang-Jin [2 ,3 ]
机构：
[1] Pukyong Natl Univ, Dept Biotechnol, Busan 608737, South Korea
[2] Korea Res Inst Biosci & Biotechnol KRIBB, Ind Biomat Res Ctr, 125 Gwahak Ro, Daejeon 34141, South Korea
[3] Korea Univ Sci & Technol UST, Dept Biomol Sci, KRIBB Sch Biosci, 217 Gajeong Ro, Daejeon, South Korea
来源：
LWT-FOOD SCIENCE AND TECHNOLOGY | 2021年 / 147卷
关键词：
Identification; Lactiplantibacillus; Lactobacillus; Intergenic spacer region; groESL; LACTOBACILLUS-PLANTARUM GROUP; PCR; DIFFERENTIATION; PARAPLANTARUM; PENTOSUS;
D O I：
10.1016/j.lwt.2021.111504
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The untranscribed DNA sequences of the intergenic spacer regions (ISR) in the groESL were analyzed to resolve the ambiguity within phylogenetically close GRAS and non-GRAS species. The sequencing results of amplified polymerase chain reaction (PCR) products using groESL-ISR-specific primers accurately distinguished L. plantarum and L. pentosus, which were not distinguished by 16 S rRNA sequences. Furthermore, the 20 selected major probiotics species were divided into several groups according to the length (22-91 bp) and homology (65-99%) of their ISR sequences, and this discrimination was consistent with core-genome-based differentiation. Therefore, ISR sequence-based species identification showed more accurate results than 16 S rRNA sequence-based identification and represented genome-based speciation.
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页数：5
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