Characterization of a novel beta-cypermethrin-degrading strain of Lactobacillus pentosus 3-27 and its effects on bioremediation and the bacterial community of contaminated alfalfa silage

被引：25

作者：

Liu, Fang ^{[1
,3
]}

Bai, Jie ^{[1
,2
,3
]}

Huang, Wenkang ^{[1
,3
]}

Li, Fuhou ^{[1
,3
]}

Ke, Wencan ^{[1
,3
]}

Zhang, Yixin ^{[1
,3
]}

Xie, Dongmei ^{[1
,3
]}

Zhang, Bo ^{[4
]}

Guo, Xusheng ^{[1
,3
]}

机构：

[1] Lanzhou Univ, Sch Life Sci, State Key Lab Grassland Agroecosyst, Lanzhou 730000, Gansu, Peoples R China

[2] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Peoples R China

[3] Lanzhou Univ, Probiot & Biol Feed Res Ctr, Lanzhou 730000, Peoples R China

[4] Anim Husb & Vet Bur Dingxi City, Dingxi 743000, Peoples R China

来源：

JOURNAL OF HAZARDOUS MATERIALS | 2022年 / 423卷

关键词：

Beta-cypermethrin; Degradation; Lactobacillus pentosus; Alfalfa silage; SMRT sequencing technology; LACTIC-ACID BACTERIA; FERMENTATION QUALITY; PESTICIDE-RESIDUES; BIODEGRADATION; STABILITY; CHLORPYRIFOS; DEGRADATION; PYRETHROIDS; WATER; MILK;

D O I：

10.1016/j.jhazmat.2021.127101

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In this study, a novel beta-cypermethrin (beta-cyp)-degrading strain Lactobacillus pentosus 3-27 (LP3-27) was screened from beta-cyp-contaminated silage. The strain could degrade 96% of beta-cyp (50 mg/L) in MSM medium after 4 d of culture, while the strain lost its degradation ability when the beta-cyp concentration reached 250 mg/L. The effects of LP 3-27 on fermentation, bacterial community, and bioremediation of contaminated alfalfa silage at two dry matter (DM) contents were studied. The results showed that inoculation with LP3-27 not only degraded beta-cyp, but also improved the fermentation quality of alfalfa silage after 60 d of ensiling. Meanwhile, L. pentosus dominated the bacterial community during ensiling in LP3-27 inoculated silages, whereas Pediococcus acidilactici was the dominant species in the control silage. LP3-27 inoculation also simplified the bacterial interaction networks of ensiled alfalfa. Beta-cyp degradation was positively correlated with L. pentosus in LP- inoculated silages, which confirmed the function of beta-cyp degradation by L. pentosus. In addition, higher beta-cyp degradation was observed in silage with 35% versus 43% DM. In summary, strain LP3-27 could be used as a candidate inoculum for bioremediation of beta-cyp-contaminated silage and to produce safe silage for animal production.

引用

页数：12