Molecular characterization of SSS139, a new satellite DNA family in sibling species of the Drosophila buzzatii cluster

被引:5
|
作者
Franco, Fernando F. [1 ]
Sene, Fabio M. [1 ]
Manfrin, Maura H. [2 ]
机构
[1] Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil
[2] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil
关键词
Drosophila buzzatii cluster; molecular evolution; repetitive DNA; satellite DNA; SSS139 satellite DNA;
D O I
10.1590/S1415-47572008000100026
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We characterized sequences of a novel SSS139 Rsal satellite DNA family in Drosophila gouveai and Drosophila seriema, two members of the Drosophila buzzatii cluster (D. repleta group). The sequences were AT-rich (69%) with a monomer unit length of about 139 bp and contained two direct subrepeats of 14 bp and 16 bp, suggesting that it might have originated by the duplication of smaller sequences. Southern and dot-blot hybridization analyses also detected SSS139 in other Drosophila buzzatii cluster species (D. koepferae, D. antonietae, D. borborema and D. serido) but not in D. buzzatii. These results agree with the marginal phylogenetic position of D. buzzatii within the D. buzzatii cluster.
引用
收藏
页码:155 / 159
页数:5
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