Mutual regulation of decapentaplegic and hedgehog during the initiation of differentiation in the Drosophila retina

被引:79
作者
Borod, ER
Heberlein, U
机构
[1] Univ Calif San Francisco, Gallo Ctr, San Francisco, CA 94110 USA
[2] Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94110 USA
[3] Univ Calif San Francisco, Program Neurosci & Dev Biol, San Francisco, CA 94110 USA
关键词
Drosophila; retina; hedgehog; atonal;
D O I
10.1006/dbio.1998.8888
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Neuronal differentiation in the Drosophila retinal primordium, the eye imaginal disc, begins at the posterior tip of the disc and progresses anteriorly as a wave. The morphogenetic furrow (MF) marks the boundary between undifferentiated anterior cells and differentiating posterior cells. Anterior progression of differentiation is driven by Hedgehog, synthesized by cells located posterior to the RIF. We report here that hedgehog (hh), which is expressed prior to the start of differentiation along the disc's posterior margin, also plays a crucial role in the initiation of differentiation. Using a temperature-sensitive allele we show that hh is normally required at the posterior margin to maintain the expression of decapentaplegic (dpp) and of the proneural gene atonal. In addition, we find that ectopic differentiation driven by ectopic dpp expression or loss of wingless function requires hh. Consistent with this is our observation that ectopic dpp induces the expression of hh along the anterior margin even in the absence of differentiation. Taken together, these data reveal a novel positive regulatory loop between dpp and hh that is essential for the initiation of differentiation in the eye disc. (C) 1998 Academic Press.
引用
收藏
页码:187 / 197
页数:11
相关论文
共 48 条
[1]   The Drosophila smoothened gene encodes a seven-pass membrane protein, a putative receptor for the hedgehog signal [J].
Alcedo, J ;
Ayzenzon, M ;
VonOhlen, T ;
Noll, M ;
Hooper, JE .
CELL, 1996, 86 (02) :221-232
[2]  
BLACKMAN RK, 1991, DEVELOPMENT, V111, P657
[3]   THE EYES ABSENT GENE - GENETIC-CONTROL OF CELL-SURVIVAL AND DIFFERENTIATION IN THE DEVELOPING DROSOPHILA EYE [J].
BONINI, NM ;
LEISERSON, WM ;
BENZER, S .
CELL, 1993, 72 (03) :379-395
[4]  
Bonini NM, 1997, DEVELOPMENT, V124, P4819
[5]  
BRAND AH, 1993, DEVELOPMENT, V118, P401
[6]   Hedgehog-dependent patterning in the Drosophila eye can occur in the absence of Dpp signaling [J].
Burke, R ;
Basler, K .
DEVELOPMENTAL BIOLOGY, 1996, 179 (02) :360-368
[7]   Hedgehog signaling in Drosophila eye and limb development - Conserved machinery, divergent roles? [J].
Burke, R ;
Basler, K .
CURRENT OPINION IN NEUROBIOLOGY, 1997, 7 (01) :55-61
[8]   TARGETED EXPRESSION OF THE SIGNALING MOLECULE DECAPENTAPLEGIC INDUCES PATTERN DUPLICATIONS AND GROWTH ALTERATIONS IN DROSOPHILA WINGS [J].
CAPDEVILA, J ;
GUERRERO, I .
EMBO JOURNAL, 1994, 13 (19) :4459-4468
[9]  
Chanut F, 1997, DEVELOPMENT, V124, P559
[10]   Dachshund and eyes absent proteins form a complex and function synergistically to induce ectopic eye development in Drosophila [J].
Chen, R ;
Amoui, M ;
Zhang, ZH ;
Mardon, G .
CELL, 1997, 91 (07) :893-903