Bioconversion of phosphatidylcholine to phosphatidylserine using immobilized enzyme mini-columns

被引：8
|
作者：
Yaqoob, M [1 ]
Nabi, A
Masoom-Yasinzai, M
机构：
[1] Univ Baluchistan, Dept Chem, Quetta, Pakistan
[2] Univ Baluchistan, Inst Biochem, Quetta, Pakistan
来源：
PROCESS BIOCHEMISTRY | 2001年 / 36卷 / 12期
关键词：
immobilization; phospholipase-D; preparation of phosphatidylserine; serine dehydratase;
D O I：
10.1016/S0032-9592(01)00156-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Phospholipase-D. immobilized on controlled porosity glass beads, was used for the preparation of phosphatidylserine. The synthesis of phosphatidylserine was monitored using immobilized mini-reactors of choline oxidase and serine dehydratase isolated from rat liver. The immobilized enzymes showed good stability, and no deterioration in enzyme activity was recorded after use for 4 months. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：1181 / 1185
页数：5
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