Microfluidic devices and true-color sensor as platform for glucose oxidase and laccase assays

被引：14
|
作者：
Demirkol, Dilek Odaci [1 ]
Dornbusch, Kay [2 ]
Feller, Karl-Heinz [2 ]
Timur, Suna [1 ]
机构：
[1] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey
[2] Univ Appl Sci, Instrumental Anal Grp, Dept Med Engn & Biotechnol, Jena, Germany
来源：
ENGINEERING IN LIFE SCIENCES | 2011年 / 11卷 / 02期
关键词：
Biosensors; Enzyme assay; Glucose oxidase; Laccase; Microfluidic devices; TRAMETES-VERSICOLOR; IMMOBILIZATION; ELECTRODE; OXYGEN; PURIFICATION; PEROXIDASE; MEDIATORS; SYSTEMS; ACID;
D O I：
10.1002/elsc.201000068
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The commonly used assay for measuring glucose oxidase (GOx) and laccase (Lac) activity, based on spectrophotometric test using 2,2'-azinobis-(3-ethylbenzothia-zoline-6-sulfonic acid) (ABTS), has been adapted to measure these enzyme activities via microfluidic devices. The effects of flow rate and concentration of substrates were examined. The developed assays allowed analyzing GOx and Lac activities in the range of 0.8-8.0 Unit (0.1-1.0 mg/mL) and 0.9-17.7 Unit (0.1-2.0 mg/mL), respectively. Moreover, repeatability tests were obtained for both enzyme assays.
引用
收藏
页码：182 / 188
页数：7
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