Improved analysis of malondialdehyde in human body fluids

被引：635
|
作者：
Jentzsch, AM
Bachmann, H
Furst, P
Biesalski, HK
机构：
[1] UNIV HOHENHEIM,INST BIOL CHEM & NUTR,D-70593 STUTTGART,GERMANY
[2] HOFFMANN LA ROCHE AG,BASEL,SWITZERLAND
来源：
FREE RADICAL BIOLOGY AND MEDICINE | 1996年 / 20卷 / 02期
关键词：
antioxidants; butylated hydroxy toluene; lipid peroxidation; malondialdehyde; thiobarbituric acid-reactive substances; free radicals;
D O I：
10.1016/0891-5849(95)02043-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The widely used TEA assay for lipid peroxidation was modified to minimize artefactual oxidative degradation of lipids during the assay. Formation of the TBA-MDA condensation product was studied with and without exclusion of oxygen, and the concentration effect of BHT addition was examined. Oxygen was depleted from the reaction mixture by extensive argon gassing. Exclusion of oxygen resulted in decreased TEARS production in plasma but not in standard solutions. High BHT concentrations resulted in a similar effect. At concentrations higher than 3 mmol/l BHT exclusion of oxygen had no additional effect. By measuring n-butanol extracts in a multititer plate reader this modified method was made suitable as a preliminary screening assay of human body fluids for lipid peroxidation.
引用
收藏
页码：251 / 256
页数：6
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