Neutralizing SARS-CoV-2 by dimeric side chain-to-side chain cross-linked ACE2 peptide mimetics

被引：3

作者：

Zhang, Yan-Ni ^{[1
,2
]}

Zhang, Yuwei ^{[3
]}

Su, Shan ^{[4
,5
]}

Zhu, Han-Ying ^{[1
,2
]}

Xu, Wei ^{[4
,5
]}

Wang, Lu ^{[3
]}

Wu, Meng ^{[1
,2
]}

Chen, Kai ^{[1
,2
]}

Yu, Fei-Qiang ^{[1
,2
]}

Xi, Tong-Kuai ^{[1
,2
]}

Zhou, Qin ^{[1
,2
]}

Xie, You-Hua ^{[4
,5
]}

Qin, Ximing ^{[1
,2
]}

Ge, Honghua ^{[1
,2
]}

Lu, Lu ^{[4
,5
]}

Qing, Jie ^{[3
]}

Fang, Ge-Min ^{[1
,2
]}

机构：

[1] Anhui Univ, Inst Phys Sci, Dept Hlth Sci, Hefei 230601, Peoples R China

[2] Anhui Univ, Inst Informat, Dept Hlth Sci, Hefei 230601, Peoples R China

[3] Southwest Med Univ, Affiliated Tradit Chinese Med Hosp, Natl Tradit Chinese Med Clin Res Base, Luzhou 646000, Peoples R China

[4] Fudan Univ, Shanghai Inst Infect Dis & Biosecur, Sch Basic Med Sci, Key Lab Med Mol Virol MOE NHC CAMS, Shanghai 200032, Peoples R China

[5] Fudan Univ, Shanghai Inst Infect Dis & Biosecur, Biosafety Level Lab 3, Shanghai 200032, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2022年 / 58卷 / 11期

基金：

中国国家自然科学基金;

关键词：

CHEMICAL-SYNTHESIS; PROTEINS; INHIBITION; ENTRY; DNA;

D O I：

10.1039/d1cc06301d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present the finding of a dimeric ACE2 peptide mimetic designed through side chain cross-linking and covalent dimerization. It has a binding affinity of 16 nM for the SARS-CoV-2 spike RBD, and effectively inhibits the SARS-CoV-2 pseudovirus in Huh7-hACE2 cells with an IC50 of 190 nM and neutralizes the authentic SARS-CoV-2 in Caco2 cells with an IC50 of 2.4 mu M. Our study should provide a new insight for the optimization of peptide-based anti-SARS-CoV-2 inhibitors.

引用

页码：1804 / 1807

页数：4

共 50 条

[1] Stability of ACE2 Peptide Mimetics and Their Implications on the Application for SARS-CoV2 Detection
Santana, Paula A. A.
Alvarez, Claudio A.
Valenzuela, Santiago
Manchego, Alberto
Guzman, Fanny
Tirapegui, Cristian
Ahumada, Manuel
BIOSENSORS-BASEL, 2023, 13 (04):
[2] Fish ACE2 is not susceptible to SARS-CoV-2
Xie, Shi-Zhe
Liu, Mei-Qin
Jiang, Ren-Di
Lin, Hao-Feng
Zhang, Wei
Li, Bei
Su, Jia
Ke, Fei
Zhang, Qi-Ya
Shi, Zheng-Li
Yang, Xing-Lou
VIROLOGICA SINICA, 2022, 37 (01) : 142 - 144
[3] ACE2: the molecular doorway to SARS-CoV-2
Miriam Marlene Medina-Enríquez
Sandra Lopez-León
José Alberto Carlos-Escalante
Zuleika Aponte-Torres
Angelica Cuapio
Talia Wegman-Ostrosky
Cell & Bioscience, 10
[4] ACE2: the molecular doorway to SARS-CoV-2
Medina-Enriquez, Miriam Marlene
Lopez-Leon, Sandra
Carlos-Escalante, Jose Alberto
Aponte-Torres, Zuleika
Cuapio, Angelica
Wegman-Ostrosky, Talia
CELL AND BIOSCIENCE, 2020, 10 (01):
[5] Soluble ACE2 in SARS-CoV-2 infection
Yeung, M. L.
NATURE REVIEWS NEPHROLOGY, 2021, 17 (5) : 297 - 297
[6] Soluble ACE2 in SARS-CoV-2 infection
Susan Allison
Nature Reviews Nephrology, 2021, 17 : 297 - 297
[7] SARS-CoV-2 infection and ACE2 inhibition
Angeli, Fabio
Reboldi, Gianpaolo
Verdecchia, Paolo
JOURNAL OF HYPERTENSION, 2021, 39 (08) : 1555 - 1558
[8] Fish ACE2 is not susceptible to SARS-CoV-2
ShiZhe Xie
MeiQin Liu
RenDi Jiang
HaoFeng Lin
Wei Zhang
Bei Li
Jia Su
Fei Ke
QiYa Zhang
ZhengLi Shi
XingLou Yang
Virologica Sinica, 2022, 37 (01) : 142 - 144
[9] Fish ACE2 is not susceptible to SARS-CoV-2
Shi-Zhe Xie
Mei-Qin Liu
Ren-Di Jiang
Hao-Feng Lin
Wei Zhang
Bei Li
Jia Su
Fei Ke
Qi-Ya Zhang
Zheng-Li Shi
Xing-Lou Yang
Virologica Sinica, 2022, (01) : 142 - 144
[10] Cross-species recognition of SARS-CoV-2 to bat ACE2
Liu, Kefang
Tan, Shuguang
Niu, Sheng
Wang, Jia
Wu, Lili
Sun, Huan
Zhang, Yanfang
Pan, Xiaoqian
Qu, Xiao
Du, Pei
Meng, Yumin
Jia, Yunfei
Chen, Qian
Deng, Chuxia
Yan, Jinghua
Wang, Hong-Wei
Wang, Qihui
Qi, Jianxun
Gao, George Fu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2021, 118 (01)

← 1 2 3 4 5 →