Proteins are tightly bound with transcribed regions of human ribosomal genes

被引：0
|
作者：
Veiko, NN [1 ]
Lyapunova, NA [1 ]
Bogush, AI [1 ]
Spitkovskii, DM [1 ]
机构：
[1] Russian Acad Med Sci, Inst Human Genet, Med Genet Res Ctr, Moscow 115478, Russia
来源：
MOLECULAR BIOLOGY | 1998年 / 32卷 / 04期
关键词：
human genome; ribosomal genes; rDNA-associated proteins;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Proteins tightly bound with DNA and protecting it from endonucleases were revealed in the transcribed regions of human ribosomal genes. In complex with rDNA, their DNA-binding domains cannot be hydrolyzed with proteinase K even under denaturing conditions. The complexes remain stable in the presence of detergents and under high ionic strength. Tightly bound proteins were detected in several DNA regions along the entire transcribed sequence of some ribosomal gene copies in all tested individuals; their presence showed no tissue specificity.
引用
收藏
页码：518 / 522
页数：5
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