Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE

Based on the theory of plug-plug kinetic capillary electrophoresis(ppKCE), the rabbit red blood cell membrane and human red blood cell membrane were used to study the kinetic parameters of the interaction between the cell membrane and drug. The rate constants of complex formation k(on), the dissociation k(off) and the binding constant K(b) between cell membrane and drugs were directly determined simultaneously. The stability of peak height and migration time was studied. The influence of pH and temperature on the kinetic parameters of the interaction between the cell membrane and drugs were examined and analyzed. The results indicate that at physiological pH value, the cell membrane showed biggest biology activeness, the k(on) and K(b) between cell membrane and drugs reached maximum value. The values of k(off), k(on) and K(b) between cell membrane and CIT, Epinastine, Gatifloxacin and Roxatidine were gained. The accuracy of ppKCE was confirmed by the time ratio method and UV-Vis absorption spectra. It could provide a new approach for the evaluation of drug efficiency, toxicity and pharmacokinetics in vivo.