Pirimicarb;
Calf thymus DNA;
Binding mode;
Spectroscopy;
Alternative least squares;
MULTIVARIATE CURVE RESOLUTION;
EVOLVING FACTOR-ANALYSIS;
NEUTRAL RED-DYE;
FLUORESCENCE PROBE;
IN-VITRO;
DEOXYRIBONUCLEIC-ACID;
BINDING-PROPERTIES;
VOLTAMMETRIC DATA;
ETHIDIUM-BROMIDE;
HUMAN URINE;
D O I:
10.1016/j.saa.2010.11.050
中图分类号:
O433 [光谱学];
学科分类号:
0703 ;
070302 ;
摘要:
The interaction between pirimicarb and calf thymus DNA in physiological buffer (pH 7.4) was investigated with the use of Neutral Red (NR) dye as a spectral probe by UV-vis absorption, fluorescence and circular dichroism (CD) spectroscopy, as well as viscosity measurements and DNA melting techniques. The results revealed that an intercalation binding should be the interaction mode of pirimicarb to DNA. CD spectra indicated that pirimicarb induced conformational changes of DNA. The binding constants of pirimicarb with DNA were obtained by the fluorescence quenching method. The thermodynamic parameters, enthalpy change (Delta H(theta)) and entropy change (Delta S(theta)) were calculated to be -52.13 +/- 12.04 kJ mol(-1) and -108.8 +/- 6.72 J mol(-1) K(-1) according to the van't Hoff equation, which suggested that hydrogen bonds and van der Waals forces might play a major role in the binding of pirimicarb to DNA. Further, the alternative least squares (ALS) method was applied to resolve a complex two-way array of the absorption spectra data, which provided simultaneously the concentration information for the three reaction components, pirimicarb, NR and DNA-NR. This ALS analysis indicated that the intercalation of pirimicarb into the DNA by substituting for NR in the DNA-NR complex. (C) 2010 Elsevier B.V. All rights reserved.
机构:
China Pharmaceut Univ, Dept Analyt Chem, Nanjing 210009, Jiangsu, Peoples R China
China Pharmaceut Univ, Minist Educ, Key Lab Drug Qual Control & Pharmacovigilance, Nanjing 210009, Peoples R ChinaChina Pharmaceut Univ, Dept Analyt Chem, Nanjing 210009, Jiangsu, Peoples R China
Ling, Xia
Zhong, Wenying
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机构:
China Pharmaceut Univ, Dept Analyt Chem, Nanjing 210009, Jiangsu, Peoples R China
China Pharmaceut Univ, Minist Educ, Key Lab Drug Qual Control & Pharmacovigilance, Nanjing 210009, Peoples R ChinaChina Pharmaceut Univ, Dept Analyt Chem, Nanjing 210009, Jiangsu, Peoples R China
Zhong, Wenying
Huang, Qi
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机构:
China Pharmaceut Univ, Dept Analyt Chem, Nanjing 210009, Jiangsu, Peoples R ChinaChina Pharmaceut Univ, Dept Analyt Chem, Nanjing 210009, Jiangsu, Peoples R China
Huang, Qi
Ni, Kunyi
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机构:
China Pharmaceut Univ, Dept Analyt Chem, Nanjing 210009, Jiangsu, Peoples R China
China Pharmaceut Univ, Minist Educ, Key Lab Drug Qual Control & Pharmacovigilance, Nanjing 210009, Peoples R ChinaChina Pharmaceut Univ, Dept Analyt Chem, Nanjing 210009, Jiangsu, Peoples R China