Spectroscopic studies of the interaction between pirimicarb and calf thymus DNA

被引:78
|
作者
Zhang, Guowen [1 ]
Hu, Xing [1 ]
Pan, Junhui [1 ]
机构
[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Pirimicarb; Calf thymus DNA; Binding mode; Spectroscopy; Alternative least squares; MULTIVARIATE CURVE RESOLUTION; EVOLVING FACTOR-ANALYSIS; NEUTRAL RED-DYE; FLUORESCENCE PROBE; IN-VITRO; DEOXYRIBONUCLEIC-ACID; BINDING-PROPERTIES; VOLTAMMETRIC DATA; ETHIDIUM-BROMIDE; HUMAN URINE;
D O I
10.1016/j.saa.2010.11.050
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
The interaction between pirimicarb and calf thymus DNA in physiological buffer (pH 7.4) was investigated with the use of Neutral Red (NR) dye as a spectral probe by UV-vis absorption, fluorescence and circular dichroism (CD) spectroscopy, as well as viscosity measurements and DNA melting techniques. The results revealed that an intercalation binding should be the interaction mode of pirimicarb to DNA. CD spectra indicated that pirimicarb induced conformational changes of DNA. The binding constants of pirimicarb with DNA were obtained by the fluorescence quenching method. The thermodynamic parameters, enthalpy change (Delta H(theta)) and entropy change (Delta S(theta)) were calculated to be -52.13 +/- 12.04 kJ mol(-1) and -108.8 +/- 6.72 J mol(-1) K(-1) according to the van't Hoff equation, which suggested that hydrogen bonds and van der Waals forces might play a major role in the binding of pirimicarb to DNA. Further, the alternative least squares (ALS) method was applied to resolve a complex two-way array of the absorption spectra data, which provided simultaneously the concentration information for the three reaction components, pirimicarb, NR and DNA-NR. This ALS analysis indicated that the intercalation of pirimicarb into the DNA by substituting for NR in the DNA-NR complex. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:687 / 694
页数:8
相关论文
共 50 条
  • [1] Spectroscopic studies on the interaction of aspartame with calf thymus DNA
    Kheirdoosh, Fahimeh
    Kashanian, Soheila
    TOXICOLOGY LETTERS, 2012, 211 : S101 - S101
  • [2] Spectroscopic studies on the interaction of pazufloxacin with calf thymus DNA
    Ling, Xia
    Zhong, Wenying
    Huang, Qi
    Ni, Kunyi
    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2008, 93 (03) : 172 - 176
  • [3] Spectroscopic Studies on the Interaction of Isatin with Calf Thymus DNA
    Kashanian, Soheila
    Khodaei, Mohammad Mehdi
    Pakravan, Parvaneh
    DNA AND CELL BIOLOGY, 2010, 29 (10) : 639 - 646
  • [4] Studies on the interaction of aminocarb with calf thymus DNA by spectroscopic methods
    Zhang, Guowen
    Hu, Xing
    Zhao, Nan
    Li, Weibo
    He, Li
    PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2010, 98 (02) : 206 - 212
  • [5] Studies on the interaction of apigenin with calf thymus DNA by spectroscopic methods
    Zhang, Shufang
    Sun, Xuejun
    Kong, Rongmei
    Xu, Mingming
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2015, 136 : 1666 - 1670
  • [6] Spectroscopic studies on the interaction of calf thymus DNA with the drug levetiracetam
    Shahabadi, Nahid
    Hadidi, Saba
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2012, 96 : 278 - 283
  • [7] Molecular Spectroscopic Studies of Farrerol Interaction with Calf Thymus DNA
    Zhang, Guowen
    Fu, Peng
    Wang, Lin
    Hu, Mingming
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2011, 59 (16) : 8944 - 8952
  • [8] Spectroscopic Studies on the Interaction of Leucine dipeptide with Calf Thymus DNA
    Cao, W. -N.
    Lv, M. -X.
    Lu, Kui
    Yang, Liu
    ISBE 2011: 2011 INTERNATIONAL CONFERENCE ON BIOMEDICINE AND ENGINEERING, VOL 3, 2011, : 374 - 377
  • [9] Molecular spectroscopic studies on the interaction of ferulic acid with calf thymus DNA
    Zhang, Shufang
    Sun, Xuejun
    Qu, Fengli
    Kong, Rongmei
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2013, 112 : 78 - 83
  • [10] Investigation on the interaction between luteolin and calf thymus DNA by spectroscopic techniques
    Zhang, Shufang
    Ling, Baoping
    Qu, Fengli
    Sun, Xuejun
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2012, 97 : 521 - 525