Hepatitis E Virus Genotype 3 Genomes from RNA-Positive but Serologically Negative Plasma Donors Have CUG as the Start Codon for ORF3

被引：14
|
作者：
Norder, Helene [1 ]
Galli, Cristina [1 ,2 ]
Magnil, Ellen [1 ]
Sikora, Per [3 ,4 ]
Ekvarn, Elisabet [5 ]
Nystrom, Kristina [1 ]
Magnius, Lars O. [6 ]
机构：
[1] Univ Gothenburg, Sahlgrenska Acad, Inst Biomed, Dept Infect Dis, Gothenburg, Sweden
[2] Univ Milan, Dept Biomed Sci Hlth, Milan, Italy
[3] Univ Gothenburg, Sahlgrenska Acad, Inst Biomed, Dept Pathol & Genet, Gothenburg, Sweden
[4] SciLife Labs, Clin Genom Gothenburg, Gothenburg, Sweden
[5] Octapharma AB, Stockholm, Sweden
[6] Ulf Lundahls Fdn, Stockholm, Sweden
来源：
INTERVIROLOGY | 2018年 / 61卷 / 02期
关键词：
HEV3; Blood donors; Mutation; Nucleotide sequence; E VIRAL-INFECTION; BLOOD-DONORS; PROTEIN; SEQUENCES; DONATIONS; VIROLOGY;
D O I：
10.1159/000491926
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Hepatitis E virus (HEV) is a pathogen that causes hepatitis worldwide. Molecular studies have identified HEV RNA in blood products although its significance is not understood. This study was undertaken to characterize HEV genomes in asymptomatic plasma donors from Sweden and Germany lacking anti-HEV. Complete open reading frames (ORFs) were obtained from HEV strains in 5 out of 18 plasma donors who tested positive for HEV RNA. All strains had CUG as the start codon of ORF3, while 147 GenBank strains all had AUG as the start codon (p < 0.0001). This substitution was found in both interrelated and unrelated strains belonging to different phylogenetic clades. The HEV strains from the seronegative plasma donors had no other substitution in common, which may be why the CUG substitution seems to ex- plain the seronegativity. (C) 2018 The Author(s) Published by S. Karger AG, Basel
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页码：96 / 103
页数：8
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