New strategy for the design, production and pre-purification of chimeric peptide with immunomodulatory activity in Salmo salar

被引：5

作者：

Munoz-Flores, Carolina ^{[1
]}

Gonzalez-Chavarria, Ivan ^{[1
]}

Sandoval, Felipe ^{[1
]}

Roa, Francisco J. ^{[1
]}

Palacios, Paulo ^{[2
]}

Astuya, Allisson ^{[3
,8
]}

Fernandez, Khaterina ^{[4
]}

Altamirano, Claudia ^{[5
]}

Romero, Alex ^{[6
,7
]}

Acosta, Jannel ^{[1
]}

Toledo, Jorge R. ^{[1
]}

机构：

[1] Univ Concepcion, Fac Ciencias Biol, Dept Fisiopatol, Biotechnol & Biopharmaceut Lab, Victor Lamas 1290,POB 160-C, Concepcion, Chile

[2] Salmones Antartica SA, Camino Angeles,Km 12,8, Biobio, Chile

[3] Univ Concepcion, Fac Nat & Oceanog Sci, Dept Oceanog, Lab Cell Culture & Marine Genom, Victor Lamas 1290,Box 160-C, Concepcion, Chile

[4] Univ Concepcion, Fac Engn, Dept Chem Engn, Lab Biomat, Victor Lamas 1290,Box 160-C, Concepcion, Chile

[5] Pontificia Univ Catolica Valparaiso, Escuela Ingn Bioquim, Lab Cult Celulares, Valparaiso 2362803, Chile

[6] Interdisciplinary Ctr Aquaculture Res INCAR, Ctr FONDAP, Concepcion, Chile

[7] Univ Austral Chile, Inst Patol Anim, Valdivia, Chile

[8] Univ Concepcion, Fac Nat & Oceanog Sci, COPAS Sur Austral, Victor Lamas 1290,Box 160-C, Concepcion, Chile

来源：

FISH & SHELLFISH IMMUNOLOGY | 2022年 / 125卷

关键词：

Antimicrobial peptide; Chimeric peptide; Salmo salar; Piscirickettsia salmonis; Cathelicidin; HOST-DEFENSE PEPTIDES; ANTIMICROBIAL PEPTIDES; AQUACULTURE; EXPRESSION; MECHANISMS; RESISTANCE; DISEASE; WILD; AMPS; SKIN;

D O I：

10.1016/j.fsi.2022.04.034

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The intensive salmon farming is associated with massive outbreaks of infections. The use of antibiotics for their prevention and control is related to damage to the environment and human health. Antimicrobial peptides (AMPs) have been proposed as an alternative to the use of antibiotics for their antimicrobial and immunomodulatory activities. However, one of the main challenges for its massive clinical application is the high production cost and the complexity of chemical synthesis. Thus, recombinant DNA technology offers a more sustainable, scalable, and profitable option. In the present study, using an AMPs function prediction methodology, we designed a chimeric peptide consisting of sequences derived from cathelicidin fused with the immunomodulatory peptide derived from flagellin. The designed peptide, CATH-FLA was produced by recombinant expression using an easy pre-purification system. The chimeric peptide was able to induce IL-1 beta and IL-8 expression in Salmo salar head kidney leukocytes, and prevented Piscirickettsia salmonis-induced cytotoxicity in SHK-1 cells. These results suggest that pre-purification of a recombinant AMP-based chimeric peptide designed in silico allow obtaining a peptide with immunomodulatory activity in vitro. This could solve the main obstacle of AMPs for massive clinical applications.

引用

页码：120 / 127

页数：8