Comparative SPR study on the effect of nanomaterials on the biological activity of adsorbed proteins

被引：12
|
作者：
Mei, Qiaohan [2 ]
Ding, Xiaorong [1 ]
Chen, Yangyang [2 ]
Hong, Jiang [2 ]
Koh, Kwangnak [3 ]
Lee, Jaebeom [3 ]
Chen, Hongxia [2 ]
Yin, Yongmei [1 ]
机构：
[1] Nanjing Med Univ, Dept Oncol, Affiliated Hosp 1, Nanjing 210029, Jiangsu, Peoples R China
[2] Shanghai Univ, Lab Biosensing Technol, Sch Life Sci, Shanghai 200444, Peoples R China
[3] Pusan Natl Univ, Dept Nanomed Engn, Miryang 627706, South Korea
来源：
MICROCHIMICA ACTA | 2012年 / 178卷 / 3-4期
基金：
中国国家自然科学基金;
关键词：
Surface plasmon resonance; Magnetic nanoparticles; BSA; Bioactivity of proteins; FLUORESCENT MAGNETIC NANOPARTICLES; OXIDE NANOPARTICLES; CARBON NANOTUBES; DEPENDENT TOXICITY; SIZE; FILM; DNA;
D O I：
10.1007/s00604-012-0837-y
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Bioactivity of proteins is evaluated to test the adverse effects of nanoparticles interjected into biological systems. Surface plasmon resonance (SPR) spectroscopy detects binding affinity that is normally related to biological activity. Utilizing SPR spectroscopy, a concise testing matrix is established by investigating the adsorption level of bovine serum albumin (BSA) and anti-BSA on the surface covered with 11-mercaptoundecanoic acid (MUA); magnetic nanoparticles (MNPs) and single-walled carbon nanotubes (SWCNTs), respectively. The immunoactivity of BSA on MNPs and SWCNT decreased by 18 % and 5 %, respectively, compared to that on the gold film modified with MUA. This indicates that MNPs cause a considerable loss of biological activity of adsorbed protein. This effect can be utilized for practical applications on detailed biophysical research and nanotoxicity studies.
引用
收藏
页码：301 / 307
页数：7
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