Cloning and nucleotide sequence of hisC gene from Corynebacterium glutamicum ATCC 13058

7The chromosomal DNA fragments of Corynebacterium glutamicum ATCC 13058 containing genes involved in histidine biosynthesis were cloned in pBluescript SK(+). To investigate the structural organization and the regulation of these genes, recombinant clones complementing E. coli hisA, hisB, hisC, hisl and hisF mutants were isolated from genomic library. A recombinant plasmid containing a 5.6-kb fragment of genomic DNA was able to complement E. coli hisA, hisB and hisF. But pCGH 13, carrying insert of 1.7-kb complemented only E. coli hisC. The genetic data indicate that these genes are clustered in a small region of the chromosome. Nucleotide sequence analysis of the 1.7-kb fragment of genomic DNA revealed one putative open reading frame which showed significant homology with the hisC gene of E. coli K-12 and Lacctococcus lactis.