The palynological characterisation of 157 honey samples from three northwest regions of Argentina (Prepuna, Yungas and Chaco) are presented to determine their botanical origin and species associations to be able to define their geographic origin. Samples were harvested during 2003-2011 and processed by means of melissopalynological conventional techniques. Onehundred and nine pollen types were identified. Representative pollen types with a frequency of occurrence greater than 50% in descending order of importance are: Salix humboldtiana, Allophylus edulis, Baccharis, Solanaceae, Eucalyptus, Schinus, Brassicaceae, Papilionoideae, Celtis, Scutia/Condalia-type and Parapiptadenia excelsa. The most important monofloral honeys are from the following: Salix humboldtiana, Scutia/Condalia-type, Allophylus edulis, Baccharis, Blepharocalyx salicifolius, Gleditsia amorphoides, Myrtaceae, Sicyos, Ziziphus mistol, Schinopsis-type, Agonandra excelsa, Anadenathera colubrina, Mimosa, all of them native species, and among introduced species are Eucalyptus, Citrus and Tithonia. Three apicultural zones and their corresponding pollen association indicators were determined: Zone I, Prepuna: Arquita trichocarpa, Prosopis ferox, Schinus areira, Baccharis, Buddleja and Mutisieae; Zone II, Yungas: Myrtaceae, Parapiptadenia excelsa, Baccharis, Salix humboldtiana, Allophylus edulis, Scutia/Condalia-type and Zanthoxylum coco; Zone III, transitional area Yungas-Chaco: Prosopis, Salix humboldtiana, Schinus, Anadenanthera colubrina and Allophylus edulis.