Association of Fc gamma receptor IIIB polymorphism with renal-allogrft in Chinese

Background: Several studies have identified Fc gamma RIIIb (Fcgr3b) polymorphisms that determine susceptibility to autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis. The objective of the study was to clarify whether Fc gamma RIIIb allele polymorphism influence susceptibility to end stage renal disease (ESRD) patients with renal transplantation. Methods: DNA fragments were amplified by PCR-SSP using genomic DNA from 172 unrelated, healthy blood donors and 171 renal recipients in our Kidney Disease Centre. We performed direct sequencing using 20 PCR products of renal recipients carrying different Fc-RIIIb forms according to the allele-specific PCR. Results: Fc-RIIIb NA1/NA2 genotype frequency showed significant difference between renal recipients and healthy controls (x(2) =19.3, P<0.005). In addition, the allele frequency also showed significant difference between the two groups. Lower frequency of genotype Fc gamma RIIIb NA1NA1 was observed in renal recipients than in healthy controls (x(2)=5.06, P=0.024). In comparison with nonrejectors, rejectors displayed no significant defference of Fc gamma RIIIb NA1/NA2 genotype frequency, as well as allele frequency. Conclusion: Fc-yRIIIb NA1/NA2 heterozygote genotype frequency was increased in ESRD patients in Chinese. The present findings showed that Fc gamma RIIIb genotype related to the disease susceptibility, although Fc-yRIIIb polymorphisms did not affect the acute rejection. (C) 2007 Elsevier B.V. All rights reserved.