Rapid measurement of total antioxidant capacity in plants

被引：189
|
作者：
Gillespie, Kelly M. [1 ]
Chae, June M. [2 ]
Ainsworth, Elizabeth A. [1 ,2 ]
机构：
[1] Univ Illinois, Inst Genom Biol, Program Physiol & Mol Plant Biol, Urbana, IL 61801 USA
[2] USDA ARS, Photosynthes Res Unit, Urbana, IL 61801 USA
来源：
NATURE PROTOCOLS | 2007年 / 2卷 / 04期
关键词：
D O I：
10.1038/nprot.2007.100
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
There is growing interest in measuring the antioxidant status of plant tissues. This protocol describes the oxygen radical absorbance capacity (ORAC) assay, which measures antioxidant inhibition of peroxyl radical-induced oxidations and is a measure of total antioxidant capacity. The assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader. Total antioxidant capacity of 64 experimental samples can easily be analyzed in 1 d. This assay is presented along with rapid assays for total phenolic content and total ascorbate content. Overall, these assays provide a general diagnostic tool of the antioxidant capacity in leaf tissue extracts.
引用
收藏
页码：867 / 870
页数：4
相关论文
共 50 条
  • [1] Rapid measurement of total antioxidant capacity in plants
    Kelly M Gillespie
    June M Chae
    Elizabeth A Ainsworth
    Nature Protocols, 2007, 2 : 867 - 870
  • [2] Measurement of total antioxidant capacity
    Young, IS
    JOURNAL OF CLINICAL PATHOLOGY, 2001, 54 (05) : 339 - 339
  • [3] Direct measurement of the total antioxidant capacity of cereal products
    Serpen, Arda
    Goekmen, Vural
    Pellegrini, Nicoletta
    Fogliano, Vincenzo
    JOURNAL OF CEREAL SCIENCE, 2008, 48 (03) : 816 - 820
  • [4] Automated measurement of total antioxidant capacity of seminal plasma
    不详
    EUROPEAN UROLOGY SUPPLEMENTS, 2005, 4 (03) : 268 - 268
  • [5] Antioxidant inhibition of oxygen radicals for measurement of total antioxidant capacity in biological samples
    Ching, SYL
    Hall, J
    Croft, K
    Beilby, J
    Rossi, E
    Ghisalberti, E
    ANALYTICAL BIOCHEMISTRY, 2006, 353 (02) : 257 - 265
  • [6] Direct measurement of the total antioxidant capacity of foods: the 'QUENCHER' approach
    Gokmen, Vural
    Serpen, Arda
    Fogliano, Vincenzo
    TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2009, 20 (6-7) : 278 - 288
  • [7] Antioxidant capacity and total phenolic content of selected plants from Turkey
    Kirca, Ayseguel
    Arslan, Esra
    INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2008, 43 (11): : 2038 - 2046
  • [8] SCREENING OF THE ANTIOXIDANT CAPACITY AND TOTAL PHENOLIC CONTENT OF THREE NATIVE PLANTS
    Butu, M.
    Rodino, S.
    Golea, D. A.
    Butu, A.
    DIGEST JOURNAL OF NANOMATERIALS AND BIOSTRUCTURES, 2014, 9 (01) : 337 - 345
  • [9] THE EFFECTS OF OPTIMIZATION METHODS ON THE DETERMINATION OF TOTAL ANTIOXIDANT CAPACITY IN SOME PLANTS
    Kar, Fatih
    Ozyurt, Dilek
    Ozturk, Birsen
    FRESENIUS ENVIRONMENTAL BULLETIN, 2019, 28 (09): : 6589 - 6595
  • [10] Total Phenolic, Flavonoids and Antioxidant Capacity of Some Medicinal and Aromatic Plants
    Nagy, Melinda
    Tofana, Maria
    Socaci, Sonia A.
    Pop, Ana Viorica
    Bors, Maria Doinita
    Farcas, Anca
    Moldovan, Ovidiu
    BULLETIN OF UNIVERSITY OF AGRICULTURAL SCIENCES AND VETERINARY MEDICINE CLUJ-NAPOCA-FOOD SCIENCE AND TECHNOLOGY, 2014, 71 (02) : 209 - 210
12345