Real-Time Monitoring of Solid-Phase PCR Using Fiber-Optic SPR

被引：29

作者：

Pollet, Jeroen ^{[1
]}

Janssen, Kris P. F. ^{[1
]}

Knez, Karel ^{[1
]}

Lammertyn, Jeroen ^{[1
]}

机构：

[1] Katholieke Univ Leuven, BIOSYST MeBioS, B-3001 Heverlee, Belgium

来源：

SMALL | 2011年 / 7卷 / 08期

关键词：

SURFACE-PLASMON RESONANCE; POLYMERASE-CHAIN-REACTION; FUNCTIONALIZED GOLD NANOPARTICLES; ENZYMATIC AMPLIFICATION; SIGNAL AMPLIFICATION; DNA INVITRO; HYBRIDIZATION; IMMUNOASSAY; ANTIBODY;

D O I：

10.1002/smll.201001984

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Real-time monitoring of solid-phase DNA amplification using a surface plasmon resonance sensor (SPR) with gold nanobead signal enhancement is presented. The sensor is applied in the quantification of a DNA aptamer and subsequent melting curve analysis. The system is suited for application in medical and food-safety diagnostics, and for the study and optimization of solid-phase polymerase chain reaction. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1003 / 1006

页数：4

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