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Genotoxicity in Filling Station Attendants Exposed to Petroleum Hydrocarbons
被引:49
|作者:
Rekhadevi, Perumalla Venkata
[1
]
Rahman, Mohammed Fazlur
[1
]
Mahboob, Mohammed
[1
]
Grover, Paramjit
[1
]
机构:
[1] Indian Inst Chem Technol, Div Biol, Toxicol Unit, Hyderabad 500607, Andhra Pradesh, India
来源:
关键词:
benzene;
comet assay;
filling stations;
gasoline;
genotoxicity;
micronucleus test;
peripheral blood lymphocytes;
toluene;
xylene;
BENZENE EXPOSURE;
DNA-DAMAGE;
OCCUPATIONAL-EXPOSURE;
URINARY METABOLITES;
TRAFFIC POLICE;
WORKERS;
LYMPHOCYTES;
BLOOD;
RISK;
GLUTATHIONE;
D O I:
10.1093/annhyg/meq065
中图分类号:
R1 [预防医学、卫生学];
学科分类号:
1004 ;
120402 ;
摘要:
Methods: The probable genetic damage was determined by comet assay and micronucleus test in peripheral blood lymphocytes (PBL) of study subjects. Air and blood sample analysis was done to estimate the benzene, toluene, and xylene (BTX) concentrations using gas chromatography-mass spectrometry. The effect of exposure on antioxidant enzymes was also studied by determining the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and rate of lipid peroxidation measured as concentration of malondialdehyde (MDA) formed. Results: The results of the present study suggest that there was a statistically significant increase in mean comet tail length (25.09 versus 10.27 mu m) and frequency of micronuclei in PBL (11.83 versus 5.83 (0)/(00;) P < 0.05) of FFSAs as compared to controls. BTX concentrations were found to be significantly higher in ambient air of petrol pumps and FFSAs showed elevated levels of these compounds in their breathing zone in comparison to controls (P < 0.05). Blood BTX levels were found to be significantly enhanced in FFSAs. SOD and GPx were significantly decreased with an increased rate of CAT and MDA in FFSAs as compared to controls. Conclusions: The results of our study suggest that exposure to BTX has the potential to cause genetic changes in the exposed subjects. The data highlight the need to maintain safety measures and intervention to minimize exposure.
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页码:944 / 954
页数:11
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