Extracellular matrix of early pulmonary fibrosis modifies the polarization of alveolar macrophage

被引:14
|
作者
Zhang, Yanwei [1 ]
Zhu, Lihua [1 ]
Hong, Jinsheng [2 ,3 ]
Chen, Chun [1 ,4 ,5 ]
机构
[1] Fujian Med Univ, Sch Pharm, Fuzhou 350122, Fujian, Peoples R China
[2] Fujian Med Univ, Affiliated Hosp 1, Canc Ctr, Dept Radiotherapy, Fuzhou 350004, Fujian, Peoples R China
[3] Fujian Med Univ, Affiliated Hosp 1, Fujian Higher Educ Inst, Key Lab Radiat Biol, Fuzhou 350004, Fujian, Peoples R China
[4] Fujian Med Univ, Fujian Key Lab Nat Med Pharmacol, Fuzhou 350122, Fujian, Peoples R China
[5] Fuzhou Univ, 1 Xuefu North Rd, Fuzhou 350122, Fujian, Peoples R China
基金
中国国家自然科学基金;
关键词
Radiation induced pulmonary fibrosis (RIPF); Extracellular matrix (ECM); Macrophage polarization; Surface roughness; Integrin; NFcB; MECHANISMS; INFLAMMATION; INHIBITION; ACTIVATION; INJURY; IL-4;
D O I
10.1016/j.intimp.2022.109179
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Macrophage polarization is modulated by many different stimuli. However, the effect of fibrotic extracellular matrix (ECM) on macrophage polarization remains unclear. In this study, a mouse model of radiation induced pulmonary fibrosis (RIPF) was established. Alveolar macrophages (AMs) were seeded on separated decellularized ECM respectively derived from early RIPF lung tissue (dECM-RIPF) and normal lung tissue (dECM-Nor), on which the polarization of AMs was examined. By way of bio-AFM analysis, a significant difference in surface roughness, but no difference in stiffness, was found between dECM-RIPF and dECM-Nor. Compared with dECM-Nor, dECM-RIPF induced a higher M1 activation and increased the levels of TNF-alpha, IL-6 and IL-18, while it showed no significant effect M2 density. Nevertheless, such effects induced by dECM-RIPF could be abrogated by the integrin pan-inhibitor. Furthermore, dECM-RIPF caused integrin-dependent activation of NFcB, and NFcB inhibitor was capable of inhibiting dECM-RIPF-induced AMs proliferation and M1 activation. Animal experi-ments showed that NFcB inhibitor alleviated RIPF mainly through inhibiting M1 activation and down-regulating the levels of inflammatory cytokines. Our results showed that differential biophysical signaling from the fibrotic ECM of early RIPF promoted AMs polarization towards a M1 phenotype via integrin-NFcB. Inhibition of M1 activation may be an attractive approach for treating RIPF.
引用
收藏
页数:11
相关论文
共 50 条
  • [1] Pirfenidone Suppresses Pulmonary Fibrosis Through Regulation of Alveolar Macrophage Polarization
    Toda, Michihito
    Mizuguchi, Shinjiro
    Minamiyama, Yukiko
    Takemura, Shigekazu
    Yamamoto, Hiroko
    Aota, Takanori
    Nishiyama, Noritoshi
    FREE RADICAL BIOLOGY AND MEDICINE, 2016, 100 : S62 - S62
  • [2] A Unique Human Alveolar Macrophage Polarization Phenotype Is Associated With Disease Progression In Idiopathic Pulmonary Fibrosis
    Nicol, L. M.
    Brittan, M.
    Marwick, J. A.
    MacKinnon, A. C.
    Mills, R.
    McFarlane, P. A.
    Wallace, W. A.
    Stewart, G.
    Simpson, J.
    Rossi, A. G.
    Howie, S. E.
    Murchison, J. T.
    Hirani, N.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2016, 193
  • [3] Macrophage-derived GPNMB trapped by fibrotic extracellular matrix promotes pulmonary fibrosis
    Wang, Jing
    Zhang, Xinxin
    Long, Min
    Yuan, Mengqin
    Yin, Juan
    Luo, Wei
    Wang, Sha
    Cai, Yu
    Jiang, Wei
    Chao, Jie
    COMMUNICATIONS BIOLOGY, 2023, 6 (01)
  • [4] Macrophage-derived GPNMB trapped by fibrotic extracellular matrix promotes pulmonary fibrosis
    Jing Wang
    Xinxin Zhang
    Min Long
    Mengqin Yuan
    Juan Yin
    Wei Luo
    Sha Wang
    Yu Cai
    Wei Jiang
    Jie Chao
    Communications Biology, 6
  • [5] The extracellular matrix and mechanotransduction in pulmonary fibrosis
    Deng, Zhenjun
    Fear, Mark W.
    Choi, Yu Suk
    Wood, Fiona M.
    Allahham, Amira
    Mutsaers, Steven E.
    Prele, Cecilia M.
    INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2020, 126
  • [6] Regulation of alveolar macrophage death in pulmonary fibrosis: a review
    Yang, Ganghao
    Yang, Yang
    Liu, Yiping
    Liu, Xiaoshu
    APOPTOSIS, 2023, 28 (11-12) : 1505 - 1519
  • [7] Regulation of alveolar macrophage death in pulmonary fibrosis: a review
    Ganghao Yang
    Yang Yang
    Yiping Liu
    Xiaoshu Liu
    Apoptosis, 2023, 28 : 1505 - 1519
  • [8] Impairment of alveolar macrophage transcription in idiopathic pulmonary fibrosis
    Ren, Ping
    Rosas, Ivan O.
    MacDonald, Sandra D.
    Wu, Hai-Ping
    Billings, Eric M.
    Gochuico, Bernadette R.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2007, 175 (11) : 1151 - 1157
  • [9] Roles of Macrophage Polarization and Macrophage-Derived miRNAs in Pulmonary Fibrosis
    Kishore, Amit
    Petrek, Martin
    FRONTIERS IN IMMUNOLOGY, 2021, 12
  • [10] Macrophage polarization and its impact on idiopathic pulmonary fibrosis
    Ge, Zhouling
    Chen, Yong
    Ma, Leikai
    Hu, Fangjun
    Xie, Lubin
    FRONTIERS IN IMMUNOLOGY, 2024, 15