Effect of Hypoxia on the Expression of Iron Regulatory Proteins 1 and the Mechanisms Involved

被引：20
|
作者：
Luo, Qian-Qian [1 ]
Wang, Dan [1 ]
Min-Yan [1 ]
Zhu, Yu Li [1 ]
机构：
[1] Nantong Univ, Dept Biochem, Inst Naut Med, Nantong 226001, Peoples R China
来源：
IUBMB LIFE | 2011年 / 63卷 / 02期
基金：
中国国家自然科学基金;
关键词：
hypoxia; hypoxia inducible factor-1; iron regulatory proteins 1; hypoxia response element; HepG2; cells; INDUCIBLE FACTOR-I; GENE-EXPRESSION; MAMMALIAN IRON; OXYGEN; HOMEOSTASIS; BINDING; HIF-1; ERYTHROPOIETIN; METABOLISM; RESPONSES;
D O I：
10.1002/iub.419
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Iron is essential for many biological processes, including oxygen delivery, and its supply is tightly regulated. Iron regulatory proteins (IRPs, IRP1 and IRP2) are master regulators of cellular iron metabolism. Hypoxia triggers a broad range of gene responses that are primarily mediated by hypoxia-inducible factor-1 (HIF-1). In this study, we have shown that hypoxia could not only upregulate the expression of hypoxia inducible factor-1 but also downregulate the expression of IRP1. However, the molecular mechanisms that govern the IRP1 response to hypoxia are not known. Herein we suggested that HIF/HRE system was an essential link between IRP1 and hypoxia. The HRE of IRP1 50-regulation regions could combine with HIF-1 in vitro. Dual-luciferase reporter assay showed that IRP1 was directly downregulated by HIF/HRE system. (C) 2011 IUBMB IUBMB Life, 63(2): 120-128, 2011
引用
收藏
页码：120 / 128
页数：9
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