The Preliminary Extraction and Separation of Rat Adipose Mesenchymal Stem Cells and Its Induced Differentiation

In this paper, the effect of injured pancreatic extract and cell growth factors on the induced differentiation of rat adipose mesenchymal stem cells to islet-like cells was investigated. The rat adipose mesenchymal stem cells were extracted and separated from adipose tissues, and were induced to differentiate by injury pancreatic extract and a variety of cytokines respectively or both of two. Insulin-positive cells were identified by dithizone staining, ELISA assay was used to detect the level of insulin expression, and the related gene expression was detected by real-time quantitative polymerase chain reaction (RT-PCR) and immunofluorescence staining. Compared with control group, the number of insulin-positive cells in the experiment groups was increased. The expressions of insulin, PDX-1, insulin in the experiment groups were significantly higher than those in the control group. The combined group more effectively induced ADMS cells into islet-like cells compared with other experimental groups, the difference was statistically significant (P < 0.05). Injured pancreatic extract combining with traditional induction can promote the adipose mesenchymal stem cells to differentiate to islet-like cells more efficiently.