Control of protein structure and function through surface recognition by tailored nanoparticle scaffolds

被引：254
|
作者：
Hong, R
Fischer, NO
Verma, A
Goodman, CM
Emrick, T [1 ]
Rotello, VM
机构：
[1] Univ Massachusetts, Dept Polymer Sci & Engn, Amherst, MA 01003 USA
[2] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 03期
关键词：
D O I：
10.1021/ja037470o
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Thioalkyl and thioalkylated oligo(ethylene glycol) (OEG) ligands with chain-end functionality were used to fabricate water-soluble CdSe nanoparticle scaffolds. Surface recognition of chymotrypsin (ChT) was achieved using these functionalized nanoparticle scaffolds, with three levels of interaction demonstrated: no interaction (OEG terminated with hydroxyl group), inhibition with denaturation (carboxylate-terminated thioalkyl ligands), and inhibition with retention of structure (carboxylate-terminated OEG). The latter process was reversible upon an increase in ionic strength, with essentially complete restoration of enzymatic activity.
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收藏
页码：739 / 743
页数：5
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