Expression and characterization of Kingle 1-5 domains of human plasminogen

The cDNA encoding Kringle 1 - 5 domains of human plasminogen (designated as K1 - 5), obtained from HepG2 by RT-PCR, was cloned into expression vector pHIL-S1. The recombinant plasmid pHIL-K1 - 5 was transformed into Pichia pastoris GS115 and the recombinant yeast was induced by methanol to express the recombinant protein. The expressed protein was purified by lysine affinity chromatography. The recombinant K1 - 5 inhibited the growth of bovine aortic endothelial cells (BAEC) stimulated by the basic fibroblast growth factor (bFGF), in a dosage-dependent manner, with a half maximal concentration of 14 mg/L. And rhK1 - 5 inhibited 47% of the BAEC migration stimulated by bFGF at the concentration of 50 mg/L. rhK1 - 5 also affected the cell cycle of BAEC and caused G(0)-G(1) arrest at the concentration of 14 mg/L.