Novel ESCC-related gene ZNF750 as potential Prognostic biomarker and inhibits Epithelial-Mesenchymal Transition through directly depressing SNAI1 promoter in ESCC

被引：28

作者：

Kong, Pengzhou ^{[1
,2
]}

Xu, Enwei ^{[1
,2
]}

Bi, Yanghui ^{[1
,2
]}

Xu, Xiaoqin ^{[1
,2
]}

Liu, Xue ^{[1
,2
]}

Song, Bin ^{[1
,2
]}

Zhang, Ling ^{[1
,2
]}

Cheng, Caixia ^{[1
,2
]}

Yan, Ting ^{[1
,2
]}

Qian, Yu ^{[1
,2
]}

Yang, Jian ^{[1
,2
]}

Ma, Yanchun ^{[1
,2
]}

Cui, Heyang ^{[1
,2
]}

Zhai, Yuanfang ^{[1
,2
]}

Zou, Binbin ^{[1
,2
]}

Liu, Xiangchen ^{[1
,2
]}

Cheng, Yikun ^{[3
]}

Guo, Shiping ^{[4
]}

Cheng, Xiaolong ^{[1
,2
]}

Cui, Yongping ^{[1
,2
]}

机构：

[1] Shanxi Med Univ, Dept Pathol, Taiyuan 030001, Shanxi, Peoples R China

[2] Shanxi Med Univ, Shanxi Key Lab Carcinogenesis & Translat Res Esop, Taiyuan 030001, Shanxi, Peoples R China

[3] Univ Calif Berkeley, Coll Letter & Sci, Berkeley, CA 94720 USA

[4] Shanxi Canc Hosp, Dept Tumor Surg, Taiyuan 030001, Shanxi, Peoples R China

来源：

THERANOSTICS | 2020年 / 10卷 / 04期

基金：

中国国家自然科学基金;

关键词：

ESCC; mutation; ZNF750; epithelial-to-mesenchymal transition; SNAI1; CANCER STATISTICS; ESOPHAGEAL; EXPRESSION; LANDSCAPE; KLF4;

D O I：

10.7150/thno.38210

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background: Cancer genomic studies have identified Zinc Finger Protein 750 (ZNF750) was a novel significantly mutated gene in esophageal squamous cell carcinoma (ESCC). This study was designed to determine the clinical value and molecular mechanisms of ZNF750 in the development of ESCC. Methods: Genomic data from 4 reported ESCC cohorts were used to analyze the mutation profile of ZNF750. Tissue microarrays were used to detect its expression in 308 ESCC samples. Furtherly, the effects of ZNF750 on proliferation, colony formation, migration and invasion were tested in ESCC cells. PCR-array, chromatin immunoprecipitation (ChIP), luciferase reporter assays, and rescue assay were used to explore the mechanism of ZNF750. Correlation of ZNF750 with its target genes was analyzed in TCGA data from various SCC types. Results: ZNF750 was frequently mutated in ESCC and the most common type was nonsense mutation. Its nucleus/cytoplasm ratio in ESCC was significantly lower than that in paired non-tumor tissues; it was an independent and potential predictor for survival in ESCC patients. Furtherly, ZNF750 knockdown significantly promoted proliferation, colony formation, migration and invasion in ESCC cells. PCR-array showed epithelial-to-mesenchymal transition (EMT) was the main biologic process affected by ZNF750. Moreover, ZNF750 directly bound to the promoter region of SNAI1 and depressed its activity. Decreased ZNF750 up-regulated SNAI1 expression and promoted EMT phenotype. SNAI1 knockdown partially reversed the malignant phenotype induced by ZNF750 knockdown. Further TCGA data analyses showed ZNF750 expression was positively correlated with E-cadherin and negatively correlated with SNAI1 N-cadherin and Vimentin in ESCC and other SCC samples. Conclusion: Our results suggest that ZNF750 may act as a tumor suppressor by directly repressing SNAI1 and inhibiting EMT process in ESCC and other types of SCC.

引用

页码：1798 / 1813

页数：16

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