KISS1R and ANKRD31 Cooperate to Enhance Leydig Cell Gene Expression via the Cytoskeletal-Nucleoskeletal Pathway

被引：1

作者：

Ricci, Giulia ^{[1
]}

Guillou, Florian ^{[2
]}

Catizone, Angela ^{[3
]}

Mele, Vincenza Grazia ^{[1
]}

Moggio, Martina ^{[1
]}

Chioccarelli, Teresa ^{[1
]}

Diano, Nadia ^{[1
]}

Meccariello, Rosaria ^{[4
]}

Pierantoni, Riccardo ^{[1
]}

Fasano, Silvia ^{[1
]}

Cobellis, Gilda ^{[1
]}

Chianese, Rosanna ^{[1
]}

Manfrevola, Francesco ^{[1
]}

机构：

[1] Univ Campania L Vanvitelli, Dipartimento Med Sperimentale, Naples, Italy

[2] Univ Tours, CNRS, IFCE, INRAE,PRC, Nouzilly, France

[3] Sapienza Univ Roma, Dipartimento Sci Anatom Istol Med Legali & Apparat, Rome, Italy

[4] Univ Napoli Parthenope, Dipartimento Sci Motorie & Benessere, Naples, Italy

来源：

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2022年 / 10卷

关键词：

kisspeptin; KISS1R; ankyrins; male fertility; Leydig cells; cytoskeletal-nucleoskeletal pathway; actin; KISSPEPTIN; ANKYRIN; SPECTRIN; RECEPTOR; GPR54; STEROIDOGENESIS; LOCALIZATION; REPRODUCTION; ORGANIZATION; ROLES;

D O I：

10.3389/fcell.2022.877270

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Kisspeptins are involved in the regulation of hypothalamic-pituitary-gonadal axis, Leydig cell functions, and testosterone secretion, acting as endogenous ligands of the KISS1 receptor. ANKRD31 protein participates in male fertility, regulating meiotic progression, and epididymal sperm maturation. Here, we show that in Leydig cells, KISS1 receptor and ANKRD31 proteins physically interact; the formation of this protein complex is enhanced by Kisspeptin-10 that also modulates F-actin synthesis, favoring histone acetylation in chromatin and gene expression via the cytoskeletal-nucleoskeletal pathway. Kp/KISS1 R system deregulation, expression impairment of cytoskeletal-nucleoskeletal mediators, Leydig gene targets, and the decreased testosterone secretion in Ankrd31(-/-) testis strongly supported our hypothesis. Furthermore, cytochalasin D treatment subverted the gene expression induction dependent on Kisspeptin-10 action. In conclusion, the current work highlights a novel role for the Kisspeptin-10 in the induction of the cytoskeletal-nucleoskeletal route, downstream a physical interaction between KISS1 receptor and ANKRD31, with gene expression activation as final effect, in Leydig cells.

引用

页数：16