Capillary electrophoresis based on continuous wave multi-photon excitation fluorescence

High performance capillary electrophoresis(HPCE) has been widely applied to the field of chemistry and life science for its advantages of high separation efficiency, short analysis times and low sample volume requirements. But how to improve the detection capability of capillary electrophoresis was ever a key problem. Multi-photon excitation (MPE) fluorescence detection could solve this problem, however this method needed expensive femtosecond mod-locked laser, which restricted the improvement of capillary electrophoresis. In this paper, we implemented a set of MPE-CE system based on continuous wave (CW), successfully exploited the CW multi-photon excitation to achieve detection of capillary electrophoresis. In our current MPE-CE system we used Ti:sapphire laser to prove MPE-CE could work in CW mode. From this point, we would substitute the laser with laser diode (LD) in future. This is really a new but also practical idea in MPE-CE system based on CW.