Comparison of Two Mycoplasma genitalium Real-Time PCR Detection Methodologies

被引：31

作者：

Twin, Jimmy ^{[1
,2
]}

Taylor, Nicole ^{[1
,2
]}

Garland, Suzanne M. ^{[1
,2
,3
,4
]}

Hocking, Jane S. ^{[5
]}

Walker, Jennifer ^{[5
]}

Bradshaw, Catriona S. ^{[6
,7
]}

Fairley, Christopher K. ^{[5
,6
]}

Tabrizi, Sepehr N. ^{[1
,2
,3
]}

机构：

[1] Royal Womens Hosp, Dept Microbiol & Infect Dis, Melbourne, Vic, Australia

[2] Murdoch Childrens Res Inst, Melbourne, Vic, Australia

[3] Univ Melbourne, Dept Obstet & Gynaecol, Melbourne, Vic 3010, Australia

[4] Royal Childrens Hosp, Dept Microbiol, Melbourne, Vic, Australia

[5] Univ Melbourne, Melbourne Sch Populat Hlth, Melbourne, Vic 3010, Australia

[6] Melbourne Sexual Hlth Ctr, Melbourne, Vic, Australia

[7] Monash Univ, Dept Epidemiol & Prevent Med, Melbourne, Vic 3004, Australia

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 03期

关键词：

QUANTITATIVE DETECTION; CHLAMYDIA-TRACHOMATIS; URETHRITIS; ASSAY; SPECIMENS; URINE; GENE; MEN; AMPLIFICATION; SENSITIVITY;

D O I：

10.1128/JCM.02328-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399).

引用

页码：1140 / 1142

页数：3

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