NOVEL KLEBSIELLA PNEUMONIAE VIRULENT BACTERIOPHAGE KPPK108.1 CAPABLE OF INFECTING THE K108 SEROTYPE STRAINS

被引：1

作者：

Evseev, P., V ^{[1
]}

Shneider, M. M. ^{[1
]}

Mikhailova, Yu, V ^{[2
]}

Shelenkov, A. A. ^{[2
]}

Yanushevich, Yu G. ^{[3
]}

Karlova, M. G. ^{[4
]}

Moiseenko, A., V ^{[4
]}

Sokolova, O. S. ^{[4
]}

Shagin, D. A. ^{[3
]}

机构：

[1] Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia

[2] Cent Res Inst Epidemiol Rospotrebnadzor, Moscow, Russia

[3] Pirogov Russian Natl Res Med Univ, Moscow, Russia

[4] Lomonosov Moscow State Univ, Moscow, Russia

来源：

BULLETIN OF RUSSIAN STATE MEDICAL UNIVERSITY | 2021年 / 06期

关键词：

bacteriophage; Klebsiela pneumoniae; capsular polysaccharide; depolymerase; PROTEIN; ALGORITHM; SOFTWARE; ACID; OCR;

D O I：

10.24075/vrgmu.2021.068

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Multidrug-resistant Klebsiela pneumoniae strains are one of the major causes of nosocomial infections caused by the antibiotic-resistant bacteria. There are different options for dealing with this threat, among which is the clinical application of bacteriophages. The study was aimed to isolate and describe a virulent bactriophage, having the potential for therapeutic use. The standard phage biology and bioinformatic methods were used, which included the advanced techniques for protein structure prediction (AlphaFold software), and electron microscopy. The virulent podovirus KPPK108.1, being the member of genus Drulisvirus, which is able to specifically infect the K. pneumoniae strains with the KL108 type capsular polysaccharide, has been isolated from the wastewater. The sequence of the bactriophage genome has been defined, the biological properties have been investigated, and the genetic features have been described.

引用

页码：67 / 74

页数：8