Replication of cowpox virus in macrophages is dependent on the host range factor p28/N1R

被引：2

作者：

Bourquain, Daniel ^{[1
]}

Schrick, Livia ^{[1
]}

Tischer, Bernd Karsten ^{[2
]}

Osterrieder, Klaus ^{[2
,3
]}

Schaade, Lars ^{[1
]}

Nitsche, Andreas ^{[1
]}

机构：

[1] Robert Koch Inst, Ctr Biol Threats & Special Pathogens 1, Seestr 10, D-13353 Berlin, Germany

[2] Free Univ Berlin, Inst Virol, Dept Vet Med, Robert von Ostertag Str 7-13, D-14163 Berlin, Germany

[3] City Univ Hong Kong, Jockey Club Coll Vet Med & Life Sci, Dept Infect Dis & Publ Hlth, Kowloon, Hong Kong, Peoples R China

来源：

VIROLOGY JOURNAL | 2021年 / 18卷 / 01期

关键词：

Orthopoxvirus; Cowpox virus; Host range; p28; RING finger; RING FINGER PROTEIN; UBIQUITIN LIGASE; POXVIRUS PROTEIN; VIRULENCE FACTOR; P-28; MOTIF; ZINC;

D O I：

10.1186/s12985-021-01640-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Zoonotic orthopoxvirus infections continue to represent a threat to human health. The disease caused by distinct orthopoxviruses differs in terms of symptoms and severity, which may be explained by the unique repertoire of virus factors that modulate the host's immune response and cellular machinery. We report here on the construction of recombinant cowpox viruses (CPXV) which either lack the host range factor p28 completely or express truncated variants of p28. We show that p28 is essential for CPXV replication in macrophages of human or mouse origin and that the C-terminal RING finger domain of p28 is necessary to allow CPXV replication in macrophages.

引用

页数：6

共 23 条

[1] Replication of cowpox virus in macrophages is dependent on the host range factor p28/N1R
Daniel Bourquain
Livia Schrick
Bernd Karsten Tischer
Klaus Osterrieder
Lars Schaade
Andreas Nitsche
Virology Journal, 18
[2] Cellular Source of the Poxviral N1R/p28 Gene Family
Robert Nicholls
Todd Gray
Virus Genes, 2004, 29 : 359 - 364
[3] Cellular source of the poxviral N1R/p28 gene family
Nicholls, RD
Gray, TA
VIRUS GENES, 2004, 29 (03) : 359 - 364
[4] Intracellular localization and protein interactions of the gene 1 protein p28 during mouse hepatitis virus replication
Brockway, SM
Lu, XT
Peters, TR
Dermody, TS
Denison, MR
JOURNAL OF VIROLOGY, 2004, 78 (21) : 11551 - 11562
[5] Complete deletion of Ectromelia virus p28 impairs virus genome replication in a mouse strain, cell type, and multiplicity of infection-dependent manner
Dong, Jianing
Paszkowski, Patrick
Kocincova, Dana
Ingham, Robert J.
VIRUS RESEARCH, 2023, 323
[6] Therapeutic p28 peptide targets essential H1N1 influenza virus proteins: insights from docking and molecular dynamics simulations
Santanu Sasidharan
Vijayakumar Gosu
Donghyun Shin
Subhradip Nath
Timir Tripathi
Prakash Saudagar
Molecular Diversity, 2021, 25 : 1929 - 1943
[7] Inhibition of hepatitis B virus replication by a dNTPase-dependent function of the host restriction factor SAMHD1
Jeong, Gi Uk
Park, Il-Hyun
Ahn, Kwangseog
Ahn, Byung-Yoon
VIROLOGY, 2016, 495 : 71 - 78
[8] Therapeutic p28 peptide targets essential H1N1 influenza virus proteins: insights from docking and molecular dynamics simulations
Sasidharan, Santanu
Gosu, Vijayakumar
Shin, Donghyun
Nath, Subhradip
Tripathi, Timir
Saudagar, Prakash
MOLECULAR DIVERSITY, 2021, 25 (03) : 1929 - 1943
[9] miR-28-3p Is a Cellular Restriction Factor That Inhibits Human T Cell Leukemia Virus, Type 1 (HTLV-1) Replication and Virus Infection
Bai, Xue Tao
Nicot, Christophe
JOURNAL OF BIOLOGICAL CHEMISTRY, 2015, 290 (09) : 5381 - 5390
[10] The IL-27 p28 Subunit Binds Cytokine-Like Factor 1 to Form a Cytokine Regulating NK and T Cell Activities Requiring IL-6R for Signaling
Crabe, Sandrine
Guay-Giroux, Angelique
Tormo, Aurelie Jeanne
Duluc, Dorothee
Lissilaa, Rami
Guilhot, Florence
Mavoungou-Bigouagou, Ulrick
Lefouili, Fouad
Cognet, Isabelle
Ferlin, Walter
Elson, Greg
Jeannin, Pascale
Gauchat, Jean-Francois
JOURNAL OF IMMUNOLOGY, 2009, 183 (12): : 7692 - 7702

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