Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods

被引：20
|
作者：
de Paula, Fabiana Martins [1 ,2 ]
Malta, Fernanda de Mello [1 ,3 ]
Marques, Priscilla Duarte [1 ]
Sitta, Renata Barnabe [1 ]
Rebello Pinho, Joao Renato [1 ,3 ]
Borges Gryschek, Ronaldo Cesar [1 ,2 ]
Chieffi, Pedro Paulo [1 ,4 ]
机构：
[1] Univ Sao Paulo, Hosp Clin, Fac Med, Inst Trop Med, Sao Paulo, SP, Brazil
[2] Univ Sao Paulo, Hosp Clin, Fac Med, Dept Molestias Infecciosas & Parasitarias, Sao Paulo, SP, Brazil
[3] Univ Sao Paulo, Hosp Clin, Fac Med, Dept Gastroenterol, Sao Paulo, SP, Brazil
[4] Fac Ciencias Med Santa Casa Sao Paulo, Sao Paulo, SP, Brazil
来源：
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2015年 / 110卷 / 02期
基金：
巴西圣保罗研究基金会;
关键词：
Strongyloides stercoralis; molecular diagnosis; conventional PCR; real-time PCR; INTESTINAL PARASITES; STERCORALIS; PCR; SCHOOLCHILDREN; CAMBODIA; HOOKWORM; SAMPLES; BRAZIL;
D O I：
10.1590/0074-02760140371
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR. The results show that molecular methods can be used as alternative tools for detecting S. stercoralis in human stool samples in tropical areas.
引用
收藏
页码：272 / 274
页数：3
相关论文
共 50 条
  • [1] Strongyloidiasis in humans: diagnostic efficacy of four conventional methods and real-time polymerase chain reaction
    Francisca Campo-Polanco, Laura
    Hernandez Sarmiento, Jose Mauricio
    Mesa, Miguel Antonio
    Velasquez Franco, Carlos Jaime
    Lopez, Lucelly
    Elena Botero, Luz
    Gutierrez Builes, Lina Andrea
    REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 2018, 51 (04) : 493 - 502
  • [2] Comparison of real-time polymerase chain reaction and conventional polymerase chain reaction methods for the rapid identification of Bacillus anthracis
    Kenar, Levent
    Ortatatli, Mesut
    Karayilanoglu, Turan
    Yaren, Hakan
    Sen, Selahattin
    MILITARY MEDICINE, 2007, 172 (07) : 773 - 776
  • [3] Comparison of Nested Polymerase Chain Reaction and Real-Time Polymerase Chain Reaction with Parasitological Methods for Detection of Strongyloides stercoralis in Human Fecal Samples
    Sharifdini, Meysam
    Mirhendi, Hossein
    Ashrafi, Keyhan
    Hosseini, Mostafa
    Mohebali, Mehdi
    Khodadadi, Hossein
    Kia, Eshrat Beigom
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2015, 93 (06): : 1285 - 1291
  • [4] Quantitative evaluation of periprosthetic infection by real-time polymerase chain reaction: a comparison with conventional methods
    Miyamae, Yushi
    Inaba, Yutaka
    Kobayashi, Naomi
    Choe, Hyonmin
    Ike, Hiroyuki
    Momose, Takako
    Fujiwara, Shusuke
    Saito, Tomoyuki
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2012, 74 (02) : 125 - 130
  • [5] Molecular Prenatal Diagnosis of -Thalassemia Using Real-Time and Multiplex Polymerase Chain Reaction Methods
    Liu, Jing-Zhong
    Yan, Mei
    Wang, Li-Rong
    Wang, Zhan-Yong
    Fan, Yin-Ping
    Xiao, Bai
    Zhou, Yan
    HEMOGLOBIN, 2008, 32 (06) : 553 - 560
  • [6] Validation of real-time polymerase chain reaction versus conventional polymerase chain reaction for diagnosis of brucellosis in cattle sera
    Abdel-Hamid, Nour H.
    Beleta, Eman I. M.
    Kelany, Mohamed A.
    Ismail, Rania I.
    Shalaby, Nadia A.
    Khafagi, Manal H. M.
    VETERINARY WORLD, 2021, 14 (01) : 144 - 154
  • [7] Diagnosis of duck circovirus infections by conventional and real-time polymerase chain reaction tests
    Fringuelli, E
    Scott, ANJ
    Beckett, A
    McKillen, J
    Smyth, JA
    Palya, V
    Glavits, R
    Ivanics, E
    Mankertz, A
    Franciosini, MP
    Todd, D
    AVIAN PATHOLOGY, 2005, 34 (06) : 495 - 500
  • [8] A comparison of sensitivities of the conventional and the real-time polymerase chain reaction methods for detection of Treponema denticola in periapical abscesses
    Ozbek, Ahmet
    Ozbek, Sekuk M.
    Ozbek, Elvan
    SAUDI MEDICAL JOURNAL, 2007, 28 (09) : 1453 - 1455
  • [9] Comparison of Analytic Methods for Quantitative Real-Time Polymerase Chain Reaction Data
    Chen, Ping
    Huang, Xuelin
    JOURNAL OF COMPUTATIONAL BIOLOGY, 2015, 22 (11) : 988 - 996
  • [10] Comparison of a conventional polymerase chain reaction with real-time polymerase chain reaction for the detection of neurotropic viruses in cerebrospinal fluid samples
    Ramamurthy, M.
    Alexander, M.
    Aaron, S.
    Kannangai, R.
    Ravi, V.
    Sridharan, G.
    Abraham, A. M.
    INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 29 (02) : 102 - 109
12345