Artificial Insemination using liquid sperm Filial Ongole Bull after sexed with different methods

The purpose of this study was to determine the success of artificial insemination (AI) using liquid sperm Filial Ongole Bulls after sexed by the percoll density gradient centrifugation (PDGC) and egg white sedimentation (EWS) sexed methods. The study materials were 135 cattle were inseminated using non sexed semen, PDGC and EWS sexed methods. Non-return rate (NRR) and the conception rate (CR) are observed. The research design used for the success of AI using chi square. The result of pregnancy examination at the age of 3 months found 21 head of pregnant cows from 45 cattle in AI with without sexed (control), 14 head of pregnant cattle from 45 cattle in the AI with EWS method, 18 head of pregnant cattle from 45 cattle in AI with PDGC method. The result showed that CR value using liquid sperm result of EWS, PDGC and without sexed were very significant (P <0.01). CR value is still higher sperm without sexed (46.67%) followed by PDGC (40%) and EWS (31.11%). The value of conception rate is lower than sexed using PDGC method. The success of AI showed better PDGC sexed method than EWS sexed method based on AI liquid sperm results with CR analysis.