Isolation of bacterial artificial chromosome DNA by means of improved alkaline lysis and double potassium acetate precipitation

被引：10
|
作者：
Villalobos, DP
Bautista, R
Cánovas, FM
Claros, MG [1 ]
机构：
[1] Univ Malaga, Dept Biol Mol & Bioquim, Inst Andaluz Biotecnol, E-29071 Malaga, Spain
[2] Univ Malaga, Fac Ciencias, E-29071 Malaga, Spain
来源：
PLANT MOLECULAR BIOLOGY REPORTER | 2004年 / 22卷 / 04期
关键词：
BAC; DNA isolation; DNA precipitation; FIGE; sodium acetate;
D O I：
10.1007/BF02772684
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This work describes an easy alkaline lysis method for isolatating bacterial artificial chromosome (BAC) DNA in sufficient quantity and quality for further manipulation without the need to use a kit. The method starts with 10 mL of culture and, by alkaline lysis only, renders up to 150 ng of DNA per milliliter of culture. This BAC DNA was successfully digested with restriction enzymes, sequenced, and subjected to PCR.
引用
收藏
页码：419 / 425
页数：7
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