Identification and characterization of Dichelobacter nodosus serogroup H from ovine footrot in India

被引：2
|
作者：
Kumar, N. Vinod [1 ]
Sreenivasulu, D. [2 ]
Karthik, A. [1 ]
机构：
[1] Coll Vet Sci, Dept Vet Microbiol, Tirupati, AP, India
[2] Coll Vet Sci, Tirupati 517502, AP, India
来源：
ANAEROBE | 2016年 / 40卷
关键词：
Andhra Pradesh; Dichelobacter nodosus; fimA gene; Footrot; Serogroup H; India; SHEEP; FLOCK;
D O I：
10.1016/j.anaerobe.2016.05.013
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A total of 56 foot swabs were collected from inter digital spaces of sheep with footrot lesions were screened for 16 rRNA of Dichelobacter nodosus by PCR. Out of the 56 samples, 38(67.85%) were found to be positive. All the positive samples were subjected to multiplex PCR targeting fimA gene for identification of serogroups of D. nodosus. Serogroup H was found along with serogroup B in 12 (55.26%) samples and with serogroup I in 8 (22.2%) samples. The serogroup H was identified for the first time from the Indian subcontinent. The phylogenetic analysis of the present sequence with the available serogroup H sequences of GenBank revealed to be in close association with the serotype H1. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：100 / 102
页数：3
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