Micropropagation of bay laurel (Daphne gnidium L)

Procedures have been developed for the micropropagation of Daphne gnidium, a shrub species of ecological interest, using explants of juvenile and adult origin. Shoot proliferation rates were significantly affected by both salt formulation and benzyladenine concentration. Best results were obtained on WP medium with 5 mu M BA. The presence of indoleacetic acid in the induction medium improved BA-induced axillary bud proliferation from juvenile explants. Rooting of shoots produced in culture was difficult, especially those of adult origin. Besides an absolute requirement for auxin, calcium concentration and the pH of the medium affected the formation of adventitious roots from regenerated shoots of D. gnidium.