Degradable microneedle patches loaded with antibacterial gelatin nanoparticles to treat staphylococcal infection-induced chronic wounds

被引：32

作者：

Lei, Xiaoling ^{[1
,4
]}

Li, Mengjin ^{[1
]}

Wang, Cheng ^{[1
]}

Cui, Pengfei ^{[1
]}

Qiu, Lin ^{[1
]}

Zhou, Shuwen ^{[1
]}

Jiang, Pengju ^{[1
]}

Li, Haihang ^{[5
]}

Zhao, Donghui ^{[1
,4
]}

Ni, Xinye ^{[2
]}

Wang, Jianhao ^{[1
]}

Xia, Jiang ^{[3
]}

机构：

[1] Changzhou Univ, Sch Pharm, Changzhou 213164, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Affiliated Changzhou No Peoples Hosp 2, Changzhou, Jiangsu, Peoples R China

[3] Chinese Univ Hong Kong, Dept Chem, Hong Kong, Peoples R China

[4] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Britton Chance Ctr Biomed Photon Wuhan Natl Lab Op, Dept Biomed Engn, Wuhan, Hubei, Peoples R China

[5] Jiangsu Trautec Med Technol Co Ltd, Changzhou 213149, Jiangsu, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2022年 / 217卷

关键词：

Degradable microneedle; Antibacterial photothermal peptide; Gelatinase-responsive release; Recombinant human type III collagen; Diabetic foot ulcer; GOLD NANOPARTICLES; BIOFILMS; DELIVERY; THERAPY;

D O I：

10.1016/j.ijbiomac.2022.07.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Infection-induced chronic wounds cause prolonged pains, a high risk of amputation, and even increased mor-tality in immunocompromised patients. Here we report an antibacterial microneedle (MN) patch, which features high degradability in biological fluids and gelatinase-responsive release of an antibacterial photothermal peptide AMP-Cypate. We first synthesize gelatin nanoparticles (GNPs) and then conjugate the AMP-Cypate to afford composite AMP-Cypate@GNPs. The proteinaceous nanoparticles can responsively release AMP-Cypate in the presence of gelatinase, an enzyme secreted specifically by Staphylococcus aureus (S. aureus). AMP-Cypate@GNPs were then deposited in the tips of MNs fabricated by PVP and recombinant human type III collagen (Col III) to devise the antibacterial MN/AMP-Cypate@GNP patches. When applied to the infection site, MNs break through the epidermis and the stratum corneum, dissolve in the infected dermis, reach the bacterial colony or biofilm, release AMP-Cypate@GNPs, and exert a gelatinase-responsive photothermal therapy under near-infrared (NIR) irradiation to kill the pathogen S. aureus. In a rat model of staphylococcal infection-induced chronic wounds mimicking the condition of diabetic foot ulcer, the antibacterial MN/AMP-Cypate@GNP patches eradiated the bacterial infection and resulted in complete healing of the wounds, proving its potential application in the treatment of chronic wound infections and diabetic foot ulcers.

引用

页码：55 / 65

页数：11