The oil quality, fatty acid composition, tocopherol contents, and phenolic compounds of oven- and microwave roasted seeds from three different poppy varieties (blue, yellow, and white) were investigated. The quantity, acidity, unsaponifiable matter, peroxide, and saponification values of oilwere generally higherin roasted seeds compared to those in raw poppy seeds (control). Total phenolics, flavonoids, anthocyanin contents, and antioxidant activity of roasted seeds were less than those of the control. Roasting decreased fatty acid contents including linoleic acid in blue, yellow, and white seeds and its contents remained 57.91, 61.91, and 64.83% incontrol oil (oil from raw seeds)but decreased to57.23, 60.78, and 64.11% in oven-roasted and 56.97, 60.08, and 60.84% in microwave-roasted seedoil, respectively. The tocopherol (alpha, beta, gamma, and delta) contents also decreased after roasting and beta and gamma-types predominated. The major phenolic compounds were vanillic, p-hydroxybenzoic, ferulic, p-coumaric,cinnamic, and protocatechuic acids. Thevanillic acid content ranged from 64.38-71.17 mg/100 g in raw seed, 41.86-49.76 mg/100 g in oven-roasted, and 43.66-56.71 mg/100 gin microwave-roasted seed. The current study revealed that poppy seeds and their oil have excellent nutritional qualities that are significantly reduced after roasting.