FREE AND TOTAL GLYCEROL DETERMINATION IN BIODIESEL SAMPLES BY ENZYMATIC METHOD WITH COLORIMETRIC DETECTION

被引：0
|
作者：
Valdez, Higor da Cal [1 ]
Amado, Roberto Salgado [1 ]
de Souza, Flavia Carvalho [1 ]
D'Elia, Eliane [1 ]
Vieira, Eduardo de Castro [2 ]
机构：
[1] Univ Fed Rio de Janeiro, Inst Quim, BR-21941909 Rio De Janeiro, RJ, Brazil
[2] CENPES PETROBRAS, BR-21941915 Rio De Janeiro, RJ, Brazil
来源：
QUIMICA NOVA | 2012年 / 35卷 / 03期
关键词：
glycerol content; biodiesel; enzymatic-colorimetric method; GAS-CHROMATOGRAPHIC DETERMINATION; DIESEL;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
FREE AND TOTAL GLYCEROL DETERMINATION IN BIODIESEL SAMPLES BY ENZYMATIC METHOD WITH COLORIMETRIC DETECTION. A method based on enzymatic activities was developed using three enzymes (glycerokinase, glycerol-3-phosphate oxidase and peroxidase) and colorimetric detection for the determination of glycerol in biodiesel. The enzymatic conversion of glycerol produces H2O2 that is eliminated by the action of peroxidase, an oxygen acceptor and 4- aminoantipirine, producing water and a colored compound, which was analyzed. This method showed good linear correlation coefficient (r = 0.9937) in the concentration range of 4.95 x 10(-5) to 3.96 x 10(-4)% (w/w) and had experimental limits of detection and quantitation of 7.10 x 10(-6) and 2.10 x 10(-5)% (w/w), respectively.
引用
收藏
页码：601 / 607
页数：7
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