Mechanism by which GABA, through its GABAA receptor, modulates glutamate release from rat cortical neurons in culture

In cortical neurons, the GABA(A) agonist, muscimol, increases: (a) basal glutamate release (with a EC50 of 99+/-7 mu M); (b) intracellular calcium and (c) membrane potential, all of these in a dose-dependent manner. These muscimol effects were specific since they were reversed by bicuculline, a GABA(A) antagonist. When the action of muscimol was measured at different KCI concentrations, an increase or decrease of the glutamate secretion was observed, depending on the KCl concentration in the medium. At low KCI concentration (5.6 mM of KCl), it depolarized, at 20 mM of KCl it had no effect, but at higher KCl concentrations (30-100 mu M of KCl), it produced a hyperpolarization in these cells. The mechanism by which the GABA-Cl--channel permits Cl- Buses. inward or outward, depending on the membrane potential. (C) 1999 Elsevier Science Ltd. All rights reserved.