High-throughput screening based on small-molecule microarrays and OI-RD microscope

被引：0

作者：

Zhu, Chenggang ^{[1
]}

Fei, Yiyan ^{[1
]}

Zhu, Xiangdong ^{[2
]}

机构：

[1] Fudan Univ, Dept Opt Sci & Engn, Shanghai, Peoples R China

[2] Univ Calif Davis, Dept Phys, Davis, CA USA

来源：

7TH IEEE INTERNATIONAL NANOELECTRONICS CONFERENCE (INEC) 2016 | 2016年

关键词：

high-throughput screening; surface chemistry; small-molecule microarrays; label-free technologies;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We developed a novel high-throughput screening platform based on small-molecule microarrays (SMMs) and oblique-incidence reflectivity difference (OI-RD) microscope. OI-RD microscope is able to monitor tens of thousands of biomolecular interaction in a single experiment without the need to label any biomolecule. We also developed surface chemistry to prepare SMMs which is able to immobilize compounds with any nucleophilic residues on phenyl-isocyanate functionalized glass slides with high efficiencies. By using OI-RD microscope and SMMs with 3,375 bioactive compounds, we screened target proteins in high-throughput and label-free mode and we found hits for respective target protein. The novel high-throughput screening platform enables target proteins with unknown structure and/ or unknown function to be effectively screened.

引用

页数：2

共 50 条

[1] High-Throughput Screening of Small-Molecule Microarrays with Label-Free Optical Scanning Microscope
Fei, Y. Y.
Landry, J. P.
Sun, Y. S.
Wang, X. B.
Luo, J. T.
Lam, K. S.
Zhu, X. D.
2009 CONFERENCE ON LASERS AND ELECTRO-OPTICS AND QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE (CLEO/QELS 2009), VOLS 1-5, 2009, : 145 - +
[2] Detection speed optimization of the OI-RD microscope for ultra-high throughput screening
Zhang, Hang
Xu, Mengjing
Li, Haofeng
Mai, Xiaohan
Sun, Jiawei
Mi, Lan
Ma, Jiong
Zhu, Xiangdong
Fei, Yiyan
BIOMEDICAL OPTICS EXPRESS, 2023, 14 (05) : 2386 - 2399
[3] Screening small-molecule compound microarrays for protein ligands without fluorescence labeling with a high-throughput scanning microscope
Fei, Yiyan
Landry, James P.
Sun, Yungshin
Zhu, Xiangdong
Wang, Xiaobing
Luo, Juntao
Wu, Chun-Yi
Lam, Kit S.
JOURNAL OF BIOMEDICAL OPTICS, 2010, 15 (01)
[4] High-Throughput Microscope for Label-Free Detection of Protein and Small-Molecule Chemical Microarrays
Fei, Y. Y.
Landry, J. P.
Sun, Y. S.
Luo, J. T.
Wang, X. B.
Lam, K. S.
Zhu, X. D.
2008 CONFERENCE ON LASERS AND ELECTRO-OPTICS & QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE, VOLS 1-9, 2008, : 62 - +
[5] High-throughput screening of small-molecule adsorption in MOF
Canepa, Pieremanuele
Arter, Calvin A.
Conwill, Eliot M.
Johnson, Daniel H.
Shoemaker, Brian A.
Soliman, Karim Z.
Thonhauser, Timo
JOURNAL OF MATERIALS CHEMISTRY A, 2013, 1 (43) : 13597 - 13604
[6] High-Throughput Protein Binding End-points and Kinetics in Microarrays Using Label-Free OI-RD Microscopes
Landry, J. P.
Sun, Y. S.
Fei, Y. Y.
Lam, K. S.
Zhu, X. D.
2008 CONFERENCE ON LASERS AND ELECTRO-OPTICS & QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE, VOLS 1-9, 2008, : 60 - +
[7] A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays
Fei, Y. Y.
Landry, J. P.
Sun, Y. S.
Zhu, X. D.
Luo, J. T.
Wang, X. B.
Lam, K. S.
REVIEW OF SCIENTIFIC INSTRUMENTS, 2008, 79 (01):
[8] Application of small molecule microarrays in high-throughput screening of protein targets
Uttamchandani, Mahesh
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2010, 240
[9] High-Throughput Screening for Small-Molecule Adiponectin Secretion Modulators
Hino, Kyosuke
Nagata, Hidetaka
Shimonishi, Manabu
Ido, Motoharu
JOURNAL OF BIOMOLECULAR SCREENING, 2011, 16 (06) : 628 - 636
[10] High-throughput screening for high-efficiency small-molecule biosynthesis
Rienzo, Matthew
Jackson, Shaina J.
Chao, Lawrence K.
Leaf, Timothy
Schmidt, Thomas J.
Navidi, Adam H.
Nadler, Dana C.
Ohler, Maud
Leavell, Michael D.
METABOLIC ENGINEERING, 2021, 63 : 102 - 125

← 1 2 3 4 5 →