Visualization of a Limonene Synthesis Metabolon Inside Living Bacteria by Hyperspectral SRS Microscopy

被引：4

作者：

Zhang, Jing ^{[1
,2
]}

Shin, Jonghyeon ^{[1
]}

Tague, Nathan ^{[1
,3
]}

Lin, Haonan ^{[1
,2
]}

Zhang, Meng ^{[2
,4
]}

Ge, Xiaowei ^{[2
,4
]}

Wong, Wilson ^{[1
,3
]}

Dunlop, Mary J. ^{[1
,3
]}

Cheng, Ji-Xin ^{[1
,2
,4
]}

机构：

[1] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[2] Boston Univ, Photon Ctr, Boston, MA 02215 USA

[3] Boston Univ, Biol Design Ctr, Boston, MA 02215 USA

[4] Boston Univ, Dept Elect & Comp Engn, Boston, MA 02215 USA

来源：

ADVANCED SCIENCE | 2022年 / 9卷 / 32期

关键词：

escherichia coli; limonene; metabolic engineering; stimulated Raman scattering; COLOCALIZATION; BIOSYNTHESIS;

D O I：

10.1002/advs.202203887

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Monitoring biosynthesis activity at single-cell level is key to metabolic engineering but is still difficult to achieve in a label-free manner. Using hyperspectral stimulated Raman scattering imaging in the 670-900 cm(-1) region, localized limonene synthesis are visualized inside engineered Escherichia coli. The colocalization of limonene and GFP-fused limonene synthase is confirmed by co-registered stimulated Raman scattering and two-photon fluorescence images. The finding suggests a limonene synthesis metabolon with a polar distribution inside the cells. This finding expands the knowledge of de novo limonene biosynthesis in engineered bacteria and highlights the potential of SRS chemical imaging in metabolic engineering research.

引用

页数：10