Coordination of divalent metal cation to amide group to form adduct ion in FAB mass spectrometry:: Implication of Zn2+ in enzymatic hydrolysis of amide bond

被引：2

作者：

Kobayashi, Hisayoshi ^{[1
]}

Morisaki, Naoko ^{[1
]}

Miyachi, Hiroyuki ^{[1
]}

Hashimoto, Yuichi ^{[1
]}

机构：

[1] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 2008年 / 56卷 / 05期

关键词：

zinc ion (Zn2+); amide group; adduct ion; FAB mass spectrometry; hydrolase;

D O I：

10.1248/cpb.56.672

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The structures of complexes between amides and metal ions were examined by FAB mass spectrometry and collision-induced dissociation (CID). Zn2+ was coordinated by the amide carbonyl oxygen atom of N-tetradecylacetamide (1). In contrast, Ca2+ and Mg2+ were coordinated by the amide group of I in both the keto and enol forms. The catalytic role of Zn2+ at the active site of the hydrolases might partly be explained by the effective attack of Zn2+ on carbonyl oxygen atom of the scissile amide group.

引用

页码：672 / 676

页数：5

共 2 条

[1] Coordination of alkali metal cation to oxygen functions to form adduct ion in fast atom bombardment mass spectrometry
Morisaki, N
Inoue, K
Kobayashi, H
Shirai, R
Morisaki, M
Iwasaki, S
TETRAHEDRON, 1996, 52 (27) : 9017 - 9024
[2] Coordination of sodium cation to an oxygen function and olefinic double bond to form molecular adduct ion in fast atom bombardment mass spectrometry
Morisaki, N
Kobayashi, H
Yamamura, Y
Morisaki, M
Nagasawa, K
Hashimoto, Y
CHEMICAL & PHARMACEUTICAL BULLETIN, 2002, 50 (07) : 935 - 940

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